Anti-RNA polymerase II CTD repeat YSPTSPS Antibody (4H8)

• Catalog number
  A1289-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  POLR2A
• Target Alternative Name
  RNA Polymerase II
• Antibody Type
  Monoclonal
• Clone
  CTD 4H8
• Species Reactivities
  Human, Yeast
• Immunogen Sequence
  10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
• Gene Id
  5430
• Appearance
  Colorless liquid
• Form
  Liquid
• Application
  ChIP, ELISA, FACS, IHC, IF, IP, ChIP-seq, WB
• Positive Control
  WB: HeLa cell lysate
• Application And Usages
  ChIP, ELISA, FACS, IHC, IF, IP, ChIP-seq WB: 1:1000
• Description
  RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  RNA   polymerase   CTD   repeat   YSPTSPS   4H8  
• Gene symbol
  LINC02488, LINC00973, LINC01929, LINC02489, LINC02560, LINC02582, ZKSCAN2-DT, SCAT1, EGFLAM-AS1
• Short name
  Anti-RNA polymerase II CTD repeat YSPTSPS Antibody (4H8)
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, RNAs are to store at -70 C
• Isotype
  Mouse IgG1
• Alternative name
  Antibody toRNA polymerizing enzyme II CTD repeat YSPTSPS (antibody to-) (4H8)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:53467
• Gene
  LINC02488
• Long gene name
  long intergenic non-protein coding RNA 2488
• Synonyms
  • CTD-2316B1
• GenBank acession
  • DR033777
  • AI929023
• Locus
  5q14.3
• Discovery year
  2017-04-27
• Pubmed identfication
  • 28415557
• Classification
  • Long intergenic non-protein coding RNAs

Gene info

• Identity
  HGNC:48868
• Gene
  LINC00973
• Long gene name
  long intergenic non-protein coding RNA 973
• Synonyms
  • CTD-2021J15.2
• Locus
  3q12.1
• Discovery year
  2013-06-19
• Entrez gene record
  100506377
• Pubmed identfication
  • 28796819
  • 29870803
• Classification
  • Long intergenic non-protein coding RNAs
• VEGA ID
  OTTHUMG00000159060

Gene info

• Identity
  HGNC:52751
• Gene
  LINC01929
• Long gene name
  long intergenic non-protein coding RNA 1929
• Synonyms
  • CTD-2171N6.1
• Locus
  18q21.2
• Discovery year
  2016-12-07
• Entrez gene record
  101927229
• Pubmed identfication
  • 28618399
• RefSeq identity
  • NR_110743
• Classification
  • Long intergenic non-protein coding RNAs
• VEGA ID
  OTTHUMG00000176191

Gene info

• Identity
  HGNC:53470
• Gene
  LINC02489
• Long gene name
  long intergenic non-protein coding RNA 2489
• Synonyms
  • CTD-2589M5.4
• Locus
  11p11.2
• Discovery year
  2017-04-27
• Entrez gene record
  101928919
• Pubmed identfication
  • 28415557
• Classification
  • Long intergenic non-protein coding RNAs

Gene info

• Identity
  HGNC:53600
• Gene
  LINC02560
• Long gene name
  long intergenic non-protein coding RNA 2560
• Synonyms
  • CTD-2619J13.13
• Locus
  19q13.43
• Discovery year
  2017-06-16
• Pubmed identfication
  • 28611953
• Classification
  • Long intergenic non-protein coding RNAs

Gene info

• Identity
  HGNC:53792
• Gene
  LINC02582
• Long gene name
  long intergenic non-protein coding RNA 2582
• Synonyms
  • CTD-2354A18.1
• Locus
  18q22.3
• Discovery year
  2017-12-20
• Entrez gene record
  100505817
• Pubmed identfication
  • 29254165
• RefSeq identity
  • NR_038340
• Classification
  • Long intergenic non-protein coding RNAs

Gene info

• Identity
  HGNC:53912
• Gene
  ZKSCAN2-DT
• Long gene name
  ZKSCAN2 divergent transcript
• Synonyms
  • CTD-2547G23.4
• Locus
  16p12.1
• Discovery year
  2018-05-24
• Pubmed identfication
  • 29780284
• Classification
  • Divergent transcripts

Gene info

• Identity
  HGNC:52930
• Gene
  SCAT1
• Long gene name
  S-phase cancer associated transcript 1
• Synonyms gene
  • LINC02081
• Synonyms gene name
  • long intergenic non-protein coding RNA 2081
• Synonyms
  • CTD-2357A8.3
  • XLOC_012582
• Locus
  17q25.3
• Discovery year
  2016-12-20
• Entrez gene record
  101928710
• Pubmed identfication
  • 28028307
  • 28125665
  • 29491376
• RefSeq identity
  • NR_110848
• Classification
  • Long non-coding RNAs with non-systematic symbols
• VEGA ID
  OTTHUMG00000177747

Gene info

• Identity
  HGNC:41169
• Gene
  EGFLAM-AS1
• Long gene name
  EGFLAM antisense RNA 1
• Synonyms gene name
  • EGFLAM antisense RNA 1 (non-protein coding)
• Synonyms
  • lncRNA-LOWEG
  • CTD-2108O9.1
• Synonyms name
  • lncRNA low expressed in gastric cancer
• Locus
  5p13.1
• Discovery year
  2011-05-24
• Pubmed identfication
  • 26537802
  • 29603493
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000162101

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products