RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody, ALEXA FLUOR 647
-
Catalog numberbs-6582R-A647
-
PricePlease ask
-
Size100ul
-
-
Long nameRNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody, ALEXA FLUOR 647 Conjugated
-
Also known asAnti-RNA polymerase II CTD repeat YSPTSPS phospho Ser5 PAb ALEXA FLUOR 647
-
CategoryConjugated Primary Antibodies
-
Conjugated withALEXA FLUOR® 647
-
Host OrganismRabbit (Oryctolagus cuniculus)
-
Target AntigenRNA polymerase II CTD repeat YSPTSPS phospho Ser5
-
SpecificityThis is a highly specific antibody against RNA polymerase II CTD repeat YSPTSPS phospho Ser5.
-
ModificationPhosphorylation
-
Modification SiteSer5
-
ClonalityPolyclonal
-
ClonePolyclonal antibody
-
Concentration1ug per 1ul
-
SourceKLH conjugated synthetic phosphopeptide derived from human RNA polymerase II CTD repeat YSPTSPS around the phosphorylation site of phospho S5.
-
Gene ID Number6193
-
Tested applicationsIF(IHC-P)
-
Recommended dilutionsIF(IHC-P)(1:50-200)
-
CrossreactivityHuman, Mouse, Rat
-
Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Background of the antigenDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
-
PurificationPurified by Protein A.
-
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
-
Excitation emission650nm/665nm
-
Synonymsp-Rpb1 CTD Ser2/Ser5; DNA directed RNA polymerase II A; p-Rpb1 CTDSer2/Ser5; DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; Polr2a; RNA pol II CTD; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPB1_HUMAN.
-
PropertiesFor facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-RNA polymerase II CTD repeat YSPTSPS phospho Ser5 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
ConjugationAlexa Fluor, ALEXA FLUOR 647
-
French translationanticorps
-
Gene target
-
Gene symbolLINC02488, LINC00973, LINC01929, LINC02489, LINC02560, LINC02582, ZKSCAN2-DT, SCAT1, EGFLAM-AS1
-
Short nameAnti-RNA polymerase II CTD repeat YSPTSPS phospho Ser5
-
TechniqueAntibody, antibodies against human proteins, antibodies for, RNAs are to store at -70 C
-
IsotypeImmunoglobulin G (IgG)
-
LabelALEXA FLUOR 647
-
Alternative nameRNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody, ALEXA FLUOR 647
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene namelong intergenic non-protein coding RNA 2488
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2017-04-27
-
Pubmed identfication
-
Classification
- Long intergenic non-protein coding RNAs
Gene info
-
Identity
-
Gene
-
Long gene namelong intergenic non-protein coding RNA 973
-
Synonyms
-
Locus
-
Discovery year2013-06-19
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Long intergenic non-protein coding RNAs
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namelong intergenic non-protein coding RNA 1929
-
Synonyms
-
Locus
-
Discovery year2016-12-07
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Long intergenic non-protein coding RNAs
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namelong intergenic non-protein coding RNA 2489
-
Synonyms
-
Locus
-
Discovery year2017-04-27
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Long intergenic non-protein coding RNAs
Gene info
-
Identity
-
Gene
-
Long gene namelong intergenic non-protein coding RNA 2560
-
Synonyms
-
Locus
-
Discovery year2017-06-16
-
Pubmed identfication
-
Classification
- Long intergenic non-protein coding RNAs
Gene info
-
Identity
-
Gene
-
Long gene namelong intergenic non-protein coding RNA 2582
-
Synonyms
-
Locus
-
Discovery year2017-12-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Long intergenic non-protein coding RNAs
Gene info
-
Identity
-
Gene
-
Long gene nameZKSCAN2 divergent transcript
-
Synonyms
-
Locus
-
Discovery year2018-05-24
-
Pubmed identfication
-
Classification
- Divergent transcripts
Gene info
-
Identity
-
Gene
-
Long gene nameS-phase cancer associated transcript 1
-
Synonyms gene
-
Synonyms gene name
- long intergenic non-protein coding RNA 2081
-
Synonyms
-
Locus
-
Discovery year2016-12-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Long non-coding RNAs with non-systematic symbols
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameEGFLAM antisense RNA 1
-
Synonyms gene name
- EGFLAM antisense RNA 1 (non-protein coding)
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year2011-05-24
-
Pubmed identfication
-
Classification
- Antisense RNAs
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
