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Anti-RNA polymerase II CTD repeat YSPTSPS (Ser5), ALEXA Fluor 594

  • Catalog number
    GENTObs-6582R-A594
  • Product name
    Anti-RNA polymerase II CTD repeat YSPTSPS (Ser5), ALEXA Fluor 594
  • Size
    100 microliters
  • Short name
    Anti-RNA polymerase II CTD repeat YSPTSPS (Ser5)
  • Type
    Conjugated Primary Antibody
  • Conjugated with
    ALEXA FLUOR® 594
  • Host organism
    Rabbit (Oryctolagus cuniculus)
  • Target Protein Peptide
    RNA polymerase II CTD repeat YSPTSPS phospho Ser5
  • Specificity
    This antibody reacts specifically with RNA polymerase II CTD repeat YSPTSPS (Ser5)
  • Modification
    Phosphorylation
  • Modification site
    Ser5
  • Clonality
    Polyclonal Antibody
  • Clone
    Polyclonal Antibodies
  • Isotype
    Immunoglobulin G (IgG)
  • Concentration
    1ug per 1ul
  • Subcellular locations
    N/A
  • Antigen Source
    KLH conjugated synthetic phosphopeptide derived from human RNA polymerase II CTD repeat YSPTSPS around the phosphorylation site of phospho S5.
  • Gene ID
    6193
  • Swiss Prot
    N/A
  • Applications
    IF(IHC-P)
  • Applications with corresponding dilutions
    IF(IHC-P)(1:50-200)
  • Cross reactive species
    Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
  • Cross Reactive Species details
    No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
  • Background information
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
  • Purification method
    Purified by Protein A.
  • Storage
    Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
  • Excitation emission
    590nm/617nm
  • Synonyms
    p-Rpb1 CTD Ser2/Ser5; DNA directed RNA polymerase II A; p-Rpb1 CTDSer2/Ser5; DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; Polr2a; RNA pol II CTD; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPB1_HUMAN.
  • Also known as
    RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody
  • Other name
    Anti-RNA polymerase II CTD repeat YSPTSPS (Ser5)
  • Advisory
    Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
  • Gene target
    RNA polymerase II CTD repeat YSPTSPS Ser5
    • Gene info
    • Identity:HGNC:53467
    • Gene:LINC02488
    • Long gene name:long intergenic non-protein coding RNA 2488
    • Synonyms:CTD-2316B1
    • Discovery year:2017-04-27
    • Pubmed identification:28415557
    • Classification:Long intergenic non-protein coding RNAs
    Gene info
    Gene info
    Gene info
    • Identity:HGNC:53470
    • Gene:LINC02489
    • Long gene name:long intergenic non-protein coding RNA 2489
    • Synonyms:CTD-2589M5.4
    • Discovery year:2017-04-27
    • Entrez gene record:101928919
    • Pubmed identification:28415557
    • Classification:Long intergenic non-protein coding RNAs
    Gene info
    • Identity:HGNC:53600
    • Gene:LINC02560
    • Long gene name:long intergenic non-protein coding RNA 2560
    • Synonyms:CTD-2619J13.13
    • Discovery year:2017-06-16
    • Pubmed identification:28611953
    • Classification:Long intergenic non-protein coding RNAs
    Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    LINC02488, LINC00973, LINC01929, LINC02489, LINC02560, LINC02582, ZKSCAN2-DT, SCAT1, EGFLAM-AS1
  • isotype filter
    • Immunoglobulin G IgG
  • Label
    ALEXA FLUOR 594
  • label filter
    • ALEXA FLUOR 594
  • technique filter
    • anti-
    • anti
  • Technique
    anti-, anti, antibody to, antibodies, RNAs are to store at -70 C
  • Alternative technique
    antibodies