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RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody, ALEXA FLUOR 488

  • Catalog number
    bs-6582R-A488
  • Product name
    RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody, ALEXA FLUOR 488
  • Size
    100ul
  • Short name
    Anti-RNA polymerase II CTD repeat YSPTSPS phospho Ser5
  • Long name
    RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody, ALEXA FLUOR 488 Conjugated
  • Also known as
    Anti-RNA polymerase II CTD repeat YSPTSPS phospho Ser5 PAb ALEXA FLUOR 488
  • Category
    Conjugated Primary Antibodies
  • Conjugated with
    ALEXA FLUOR® 488
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    RNA polymerase II CTD repeat YSPTSPS phospho Ser5
  • Specificity
    This is a highly specific antibody against RNA polymerase II CTD repeat YSPTSPS phospho Ser5.
  • Modification
    Phosphorylation
  • Modification Site
    Ser5
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Isotype
    Immunoglobulin G (IgG)
  • Concentration
    1ug per 1ul
  • Source
    KLH conjugated synthetic phosphopeptide derived from human RNA polymerase II CTD repeat YSPTSPS around the phosphorylation site of phospho S5.
  • Gene ID Number
    6193
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Human, Mouse, Rat
  • Cross reactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Background of the antigen
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
  • Purification
    Purified by Protein A.
  • Storage conditions
    Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
  • Excitation emission
    499nm/519nm
  • Synonyms
    p-Rpb1 CTD Ser2/Ser5; DNA directed RNA polymerase II A; p-Rpb1 CTDSer2/Ser5; DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; Polr2a; RNA pol II CTD; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPB1_HUMAN.
  • Gene target
    RNA polymerase II CTD repeat YSPTSPS Ser5
    • Gene info
    • Identity:HGNC:53467
    • Gene:LINC02488
    • Long gene name:long intergenic non-protein coding RNA 2488
    • Synonyms:CTD-2316B1
    • Discovery year:2017-04-27
    • Pubmed identification:28415557
    • Classification:Long intergenic non-protein coding RNAs
    Gene info
    Gene info
    Gene info
    • Identity:HGNC:53470
    • Gene:LINC02489
    • Long gene name:long intergenic non-protein coding RNA 2489
    • Synonyms:CTD-2589M5.4
    • Discovery year:2017-04-27
    • Entrez gene record:101928919
    • Pubmed identification:28415557
    • Classification:Long intergenic non-protein coding RNAs
    Gene info
    • Identity:HGNC:53600
    • Gene:LINC02560
    • Long gene name:long intergenic non-protein coding RNA 2560
    • Synonyms:CTD-2619J13.13
    • Discovery year:2017-06-16
    • Pubmed identification:28611953
    • Classification:Long intergenic non-protein coding RNAs
    Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    LINC02488, LINC00973, LINC01929, LINC02489, LINC02560, LINC02582, ZKSCAN2-DT, SCAT1, EGFLAM-AS1
  • isotype filter
    • Immunoglobulin G IgG
  • Label
    ALEXA FLUOR 488
  • label filter
    • ALEXA FLUOR 488
  • technique filter
    • Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for, RNAs are to store at -70 C
  • Alternative technique
    antibodies