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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from Toc75 protein sequence of Pisum sativum (Q43715)
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS06 150 | anti-Toc75 (monocots and dicots)AS07 239 | anti-Toc159 (guinea pig antibody)AS07 236 | anti-Toc33 (hen antibody)
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Recommended dilutions for use
1:1000 with standard ECL (WB)
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Molecular weight expected аpparent
88 | 75 kDa (for Pisum sativum)
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Verified reactivity
Pisum sativum, Synechocystis PCC6803
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Possible reactivity
n.a.
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
to be added when available
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References
Ulrich et al. (2012). Chloroplast β-Barrel Proteins Are Assembled into the Mitochondrial Outer Membrane in a Process That Depends on the TOM and TOB Complexes. J. Biol. Chem. August 10.
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Scientific context
Import of protein precurors into chloroplasts occurs via translocon complexes at the outer (Toc/TOC complex) and inner (Tic/TIC complex) envelope membrane. The major components of the Toc (TOC) complex in plants are Toc75, Toc34 and Toc159 (formerly Toc86). Toc75 (OEP75) forms the main import pore. It is an abundant protein in the outer envelope membrane of chloroplasts from expanding tissues but it is also found in plastids of roots, stems and flowers. Alternative names: 75 kDa translocon at the outer-envelope membrane of chloroplasts, import intermediate-associated protein of 75 kDa, 75 kDa chloroplast outer envelope protein,
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Notes
None
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Protein number
Q43715
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TAIR number
Refer to NCBI
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Description
Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
