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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from available plant sequences of Tic40 including Arabidopsis thaliana At5g16620
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution please add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunofluorescence (IF)
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Connected products
AS10 709-10 | anti-Tic40, smaller antibody packAS06 150 | anti-Toc75AS08 293 | anti-Tic110Plant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 2500 with standard ECL (WB),
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Molecular weight expected аpparent
48 | 45 kDa (Arabidopsis thaliana)
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Verified reactivity
Arabidosps thaliana, Catharantus roseus, Nicotiana tabacum, Oryza sativa, Vitis vinifera
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Possible reactivity
dicots: Pisum sativum, Ricinus communis;monocots, trees: Picea sitchenis, Populus trichocarpa, moss: Physcomitrella patens
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No reactivity
Chlamydomonas reinhardtii
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Supplementary information
to be added when available
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References
Yang et al. (2016). OsCLT1, a CRT-like transporter 1, is required for glutathione homeostasis and arsenic tolerance in rice. New Phytol. 2016 Feb 25. doi: 10.1111/nph.13908 Yang et al. (2016). LIR1 regulates light-dependent attachment of LFNR to the thylakoid membrane in plants. Plant Cell. 2016 Mar 3. pii: tpc.01027.2015. Román et al. (2015). Non-redundant contribution of the plastidial FAD8 ω-3 desaturase to glycerolipid unsaturation at different temperatures in Arabidopsis. Mol Plant. 2015 Jun 13. pii: S1674-2052(15)00267-1. doi: 10.1016/j.molp.2015.06.004. Yin et al. (2014). The membrane proteome of stroma thylakoids from Arabidopsis thaliana studied by successive in-solution and in-gel digestion. Physiol Plant. 2014 Nov 17. doi: 10.1111/ppl.12308. Wang et al. (2014). Maintenance of Chloroplast Structure and Function by Overexpression of the Rice MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE Gene Leads to Enhanced Salt Tolerance in Tobacco. Plant Physiol. 2014 May 19;165(3):1144-1155. Brillouet et al. (2013).The tannosome is an organelle forming condensed tannins in the chlorophyllous organs of Tracheophyta. Ann Bot. Sep. 11. (Vitis vinifera, immunofluorescence)
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Scientific context
Tic40 is a component of the inner envelope membrane import complex (TIC) of plant chloroplasts. Tic40 has been proposed to function as a co-chaperone in the stromal chaperone complex that facilitates protein translocation across the inner membrane. Tic40 can be used as a cellular [compartment marker] for chloroplast inner envelope membrane.
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Notes
cellular [complartment marker] of chloroplast membrane
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Protein number
Q9FMD5
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TAIR number
AT5G16620
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Description
Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
