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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide conserved in available Toc75 (OPE75) sequences from monocots and dicots including Arabidopsis thaliana (Q9STE8, At3g46740). The Arabidopsis genome contains three Toc75-related sequences, termed atTOC75-I, atTOC75-III, and atTOC75-IV with atToc75-III being the most expressed one.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS08 292 | anti-Toc75 (Pisum sativum)AS07 239 | anti-Toc159 (guinea pig antibody)Plant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 1000 - 1: 5000 with standard ECL (WB)
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Molecular weight expected аpparent
89 kDa (with transit peptide) | 75 kDa (for Arabidopsis thaliana)
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Verified reactivity
Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Pisum sativum
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Possible reactivity
Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Citrus clementina, Citrus sinensis, Coffea canephora, Eucalyptus grandis, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Medicago truncatula, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Populus sp., Prunus persica, Zea mays, magnoliophyta: Citrus sp., moss: Physcomitrella patens
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No reactivity
Chlamydomonas reinhardtii
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Supplementary information
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References
Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843. Wang et al. (2014). Maintenance of chloroplast structure and function by overexpression of the OsMGD gene leads to enhanced salt tolerance in tobacco. Plant Physiol. 2014 May 19. pii: pp.114.238899. Brillouet et al. (2013).The tannosome is an organelle forming condensed tannins in the chlorophyllous organs of Tracheophyta. Ann Bot. Sep. 11. (Vitis vinifera, immunofluorescence)
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Scientific context
Import of protein precurors into chloroplasts occurs via translocon complexes at the outer (Toc/TOC complex) and inner (Tic/TIC complex) envelope membrane. The major components of the Toc (TOC) complex in plants are Toc75, Toc34 and Toc159 (formerly Toc86). Toc75 (OEP75) forms the main import pore. It is an abundant protein in the outer envelope membrane of chloroplasts from expanding tissues but it is also found in plastids of roots, stems and flowers.
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Notes
Toc75 can be used as a cellular [compartment marker] of chloroplast outer envelope membrane.
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Protein number
Q9STE8
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TAIR number
AT3G46740
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Description
Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
