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Available ordering format
Lyophilized in PBS pH 8.0 with 0.02% sodium azide
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Immunogen
soluble domain of Pisum sativum Toc34 overexpressed in E.coli and purified by His-tag affinity purification. Overexpressed Toc34 is lacking the transmembrane C-terminal domain.
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Raised in
Hen
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgY
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), ELISA (ELISA), immunoprecipitation (IP)
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Connected products
AS07 211 | anti-Toc34 | OEP34, chloroplast outer envelope membrane translocon complex protein AS07 237 | anti- Toc34 | OEP34, chloroplast outer envelope membrane translocon complex protein AS07 238 | anti-Toc34 | OEP34, chloroplast outer envelope membrane translocon complex protein
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Recommended dilutions for use
1 : 100 000 with alkaline phosphatase (WB)
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Molecular weight expected аpparent
29 kDa
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Verified reactivity
Pisum sativum, Spinacia oleracea
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Possible reactivity
n.a.
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No reactivity
Arabidopsis thaliana
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Supplementary information
to be added when available
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References
Reddick et al. (2007). In vitro comparative kinetic analysis of the chloroplast Toc GTPases. J Biol Chem. 2007 Apr 13;282(15):11410-26.
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Scientific context
Translocon at the outer envelope membrane of chloroplasts - Toc consists of three components: Toc34, Toc86, and Toc75. Toc 75 forms an outer membrane porin that may serve as the outer membrane proteinconducting channel.
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Notes
Pisum sativum Toc34 (chloroplast form) is Arabidopsis thaliana Toc33 that is expressed in the green leaves. There is a slight MW shift between PsToc34 and PsToc33 which can be used to identify the dual expression however these two forms of the protein are differentially expressed.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Description
Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
