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Available ordering format
Lyophilized
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Immunogen
maize cytochrome f purified from chloroplasts, including a final gel purification on a denaturing gel. protein used to elicit this antibody is conserved in Arabidopsis thaliana cyt f P56771, AtCg00540
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunogold (IG)
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Connected products
AS06 119 | anti-cytochrome f (PetA) rabbit antibodyAS07 231 | anti-cytochrome f (PetA) rabbit antibody
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Recommended dilutions for use
1: 2500- 1:5000 with standard ECL (WB), 1:120 (IG)
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Molecular weight expected аpparent
31-32 kDa
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Verified reactivity
Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Picea abies, Thermosynechococcus elongatus, Solanum lycopersicum, Zea mays
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Possible reactivity
Brachypodium distachyon, Oryza sativa, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
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References
Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113 Pavlovic et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009. Liu and Last (2015). A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. Plant J. 2015 Jun;82(5):731-43. doi: 10.1111/tpj.12845. Epub 2015 Apr 29. Dahal et al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479. Renato et al. (2014). Tomato fruit chromoplasts behave as respiratory bioenergetic organelles during ripening. Plant Physiol. 2014 Aug 14. pii: pp.114.243931. (western blot, immunogold)
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Scientific context
Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c-™-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.
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Notes
None
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Protein number
P56771
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TAIR number
ATCG00540
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