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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana cytochrome c protein sequence, UniProt:D7KMK0 (C-1) D7LY03 (C-2) , TAIR: At1g22840 (Cytc1) and At4g10040 (Cytc2)
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 50 µl of sterile water
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Storage condition
store lyophilized at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles and store at -80°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
Western blot (WB)
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Connected products
AS04 052 | anti-COXII hen antibodyAS04 053A | anti-COXII rabbit antibodyAS06 151 | anti-COXIIb antibodysecondary antibodies
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Recommended dilutions for use
1: 5000 with standard ECL (WB)
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Molecular weight expected аpparent
12,5 | 14 kDa for A. thaliana
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
cytc1 and cytc2 from following species: A. theoprasi, B. napus, B. oleracea, C. maxima, Ch. reinhardtii (peptide target partially conserved), L. esculentum, Lupinus luteus, M. truncatula, O. sativa, Ostreococcus (peptide target partially conserved), P. aurea, Physcomitrella patens, R. comunis, S. nigra, V. vinifera
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No reactivity
Arabidopsis thaliana CytC6
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Supplementary information
The presence of cytochrome c in the cysotol is a marker of PCD (programmed cell death).
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References
to be added when available, antibody release in November 2014
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Scientific context
Cytochrome c is located in inner mitochondrial membrane. It is a small heme protein which, unlike other cytochromes, is highly soluble. This protein is an essential component of the electron transport chain, where it undergoes oxidation and reduction without binding oxygen.
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Notes
None
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Protein number
D7KMK0 , D7LY03
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TAIR number
AT1G22840, At4g10040
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