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Available ordering format
Lyophilized
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Immunogen
GST fusion to cytochrome f from Chlamydomonas reinhartii P23577
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS08 306 | anti-cytochrome f (with reactivity to cyanobacteria)Collection of antibodies to Arabidopsis proteinsCollection of antibodies to Chlamydomonas proteinsPlant and algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1:2000 with alkaline phosphatase, 1: 50 000 with ECL (WB)
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Molecular weight expected аpparent
34 | 31-32 kDa
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Verified reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Hordeum vulgare, Nannochloropsis gaditana, Nicotiana tabacum, Physcomitrella patens, Selaginella martensi
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Possible reactivity
Citrus x limon, green algae, Zea mays
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Reaction with cyanobacteria: Synechocystis 6803 and Synechococcus 7942 possible to obtain on total cell extract when using antibody at 1: 500 and longer exposure time.
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References
Ferroni et al. (2016). Light acclimation in the lycophyte Selaginella martensii depends on changes in the amount of photosystems and on the flexibility of the light-harvesting complex II antenna association with both photosystems. New Phytol. 2016 Apr 5. doi: 10.1111/nph.13939. Suorsa et al. (2015). Light acclimation involves dynamic re-organisation of the pigment-protein megacomplexes in non-appressed thylakoid domains. Plant J. 2015 Aug 29. doi: 10.1111/tpj.13004. Charuvi et al. (2015). Photoprotection Conferred by Changes in Photosynthetic Protein Levels and Organization during Dehydration of a Homoiochlorophyllous Resurrection Plant. Plant Physiol. 2015 Apr;167(4):1554-65. doi: 10.1104/pp.114.255794. Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741. Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.
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Scientific context
Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c-™-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.
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Notes
contains 0.02 % sodium azide as preservative
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Protein number
P23577
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TAIR number
ATCG00540
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