Cyt b6 / PetB, Thylakoid membrane cytochrome b6 protein, N terminal

• Catalog number
  AS03 034
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• Size
  100 µl

• Available ordering format
  Lyophilized
• Immunogen
  KLH-conjugated peptide chosen from N terminal sequence of cytb6 conserved in Arabidopsis thaliana P56773 and Chlamydomonas reinhardtii NP_958365.1
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 100 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB)
• Connected products
  AS08 330 | anti-PetC rabbit antibodyAS06 202 | anti-cytochrome c6Collection of antibodies to Arabidopsis proteinsCollection of antibodies to Chlamydomonas proteinsPlant and algal protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 10 000 with standard ECL (WB)
• Molecular weight expected аpparent
  23-25 kDa
• Verified reactivity
  Arabidopsis thaliana, Chlamydomonas reinhardtii, Flaveria bidentis, Nicotiana tabacum, Thalassiosira weisflogii and Thalassiosira pseudonana
• Possible reactivity
  Hordeum vulgare,, Pisum sativum, Oryza sativa, Populus alba, Salvia miltiorrhiza, Spinacia oleracea, Zea mays
• No reactivity
  Synechocystis sp. 6803, Heterosigma akashiwo
• Supplementary information
  n.a.
• References
  Ishikawa et al. (2016). NDH-Mediated Cyclic Electron Flow Around Photosystem I is Crucial for C4 Photosynthesis. Plant Cell Physiol. 2016 Aug 6. pii: pcw127. [Epub ahead of print] Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675. Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741.
• Scientific context
  Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c-™-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.
• Notes
  None
• Protein number
  P56773 , 41179010
• TAIR number
  ATCG00720
• Hyperlink
• Description
  Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

• Gene target
  Cyt   PetB   Thylakoid   membrane   cytochrome   protein   terminal  
• Short name
  Cyt b6 / PetB, Thylakoid cytochrome b6 protein, N terminal
• Alternative name
  Cyt b6 / PetB, Thylakoid membrane cytochrome b6 protein, N terminal

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