Human IgM Fab mu fragment (myeloma)-FITC conjugate (isotype control)

  • Catalog number
    20007-4-F
  • Price
    Please ask
  • Size
    100 ug
  • Stock availability
    Available
  • Category
    Isotype Control
  • Antibody type
    N/A
  • Antibody host
    N/A
  • Antibody conjugate
    FITC
  • Technical datasheet
    Contact Gentaur to request the datasheet or ask our specialists for more information.
  • Notes
    The Human IgM Fab mu fragment (myeloma)-FITC conjugate (isotype control) is manufactured for Research Use Only or for diagnostics purposes.
  • Description
    Isotype or positive controls by peptides, antibodies and deactivated samples.
  • Properties
    Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent, This adi Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. adi FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen, Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Conjugation
    Anti-FITC Antibody
  • Group
    isotypes
  • Gene target
  • Short name
    IgM Fab mu (myeloma)-FITC conjugate (isotype control)
  • Technique
    Control, Isotype, FITC, Fab, IgM, controls, fabs, Fluorescein, IgMs, Isotypes and controls
  • Isotype
    IgM,  Fab fragment, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common. IgM
  • Label
    FITC
  • Species
    Human, Humans
  • Alternative name
    H. sapiens Immunoglobulin M Fab mu fragment (myeloma)-fluorecein conjugate (isoform reference)
  • Alternative technique
    control, controls, fluorescine, seconairy, immunoglobulin-m
  • Tissue
    control
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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