Human IgM protein Fab mu FITC
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Catalog number
65C-CH0920
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Price
Please ask
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Size
1 mg
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Category
Immunoglobulins
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Antibody Subtype
Conjugated Immunoglobulins
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Area of research
Immunology
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Reactivity
Human
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Conjugate Tag
FITC
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Method of Purification
Human IgM protein (Fab mu) (FITC) was purified by delipidation, salt fractionation, ion exchange chromatography and dialysis.
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Source
Human serum
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Form Buffer
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA and 0.01% NaN3. I mMunoglobulin and protease free.
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Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
IF
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Usage Recommendations
Optimal conditions to be determined by end user
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Properties
Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent, This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Conjugation
Anti-FITC Antibody
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Gene target
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Gene symbol
AP4M1, PRKD1, AP1M1
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Short name
IgM protein Fab mu FITC
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Technique
FITC, Fab, IgM, fabs, Fluorescein, IgMs
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Isotype
IgM, Fab fragment, IgM
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Label
FITC
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Species
Human, Humans
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Alternative name
Human IgM protein (Fab mu) (FITC) conjugate
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Alternative technique
fluorescine, seconairy, immunoglobulin-m
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Gene info
Gene info
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Identity
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Gene
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Long gene name
protein kinase D1
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Synonyms gene
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Synonyms gene name
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Synonyms
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Locus
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Discovery year
1994-05-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Pleckstrin homology domain containing
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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