Rabbit IgG Isotype Control Polyclonal Antibody FITC Conjugate

  • Catalog number
  • Supplier
  • Price
    364.2 USD
  • Size
  • Category
  • Concentration
    0.5 mg/ml in 1X PBS with 0.05% sodium azide
  • Form
  • Conjugation
    FITC Conjugate
  • Clone
    Polyclonal antibody
  • Recognised antigen
    Rabbit IgG Isotype Control FITC Conjugate
  • Host animal
    Rabbit (Oryctolagus cuniculus)
  • Clonality
    Polyclonal (rabbit origin)
  • Species reactivity
    isotype control Igs
  • Tested applications
  • Recommended dilutions
    FACS: assay dependent,IHC: assay dependent,IF: assay dependent
  • Notes
    Generally to be used at the same concentration as the sample being controlled for.
  • Added buffer
    1X Phosphate Buffered Saline (PBS), pH 7.4
  • Intented use
    This rabbit isotype control antibodyis to be used only for research purposes and not for diagnostics..
  • Gene ID
  • Purity
    Purified by protein A Affinity Chromatography
  • Description
    Isotype control antibodies are negative controls used to measure non-specific binding or background levels of a primary antibody in different cell types. They are not specific to any target in a cell, yet retain all non-specific characteristics of the antibodies they control.
  • Immunogen
    This is purified native rabbit sera and as such there was no immunogen.
  • Storage
    Store the Rabbit IgG isotype control antibody at 4oC. Do not freeze; protect from light.
  • Properties
    If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This NJS poly Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. NJS poly FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Additional description
    Isotype or positive controls by peptides, antibodies and deactivated samples.
  • Additional conjugation
    Anti-FITC Antibody
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by NJS poly. Rabbit antibodies are very stable and can be stored for several days at room temperature. NJS poly adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Group
    isotypes, Polyclonals and antibodies
  • Latin name
    Oryctolagus cuniculus
  • French translation
  • Gene target
  • Gene symbol
  • Short name
    IgG Isotype Control
  • Technique
    Polyclonal, Antibody, Control, Isotype, Rabbit, FITC, IgG, antibodies against human proteins, antibodies for, controls, Fluorescein, IgGs, Isotypes and controls, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Rabbit IgG, IgG
  • Label
  • Alternative name
    Rabbit IgG Isotype Control FITC Conjugate
  • Alternative technique
    polyclonals, antibodies, control, controls, rabbit-anti, fluorescine, immunoglobulins
  • Tissue
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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