Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit

• Catalog number
  K678-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method-Absorbance (450 nm) • Applications- - Measurement of α-Ketoglutarate dehydrogenase activity in various tissues/cells - Analysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism in various cell types
• Detection Method
  Absorbance (450 nm)
• Applications
  • Measurement of α-Ketoglutarate dehydrogenase activity in various tissues/cells • Analysis of cell signaling pathways such as citrate acid cycle, lysine degradation or tryptophan metabolism in various cell types
• Sample Type
  • Animal tissues: liver, heart, muscle, etc. • Purified mitochondria • Cell culture: Adherent or suspension cells
• Features Benefits
  • Simple, rapid & convenient • can measure α-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  α-Ketoglutarate Dehydrogenase (α-KGDH) (EC 1.2.4.2) is a key enzyme in the citric acid cycle. It forms an enzyme complex with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3). α-KGDH converts α-ketoglutarate into succinyl-CoA in the presence of NAD and CoA. It is highly regulated by intracellular ATP/ADP and NADH/NAD ratios and calcium. In humans, decreased KGDH activity can lead to neurodegenerative diseases such as Alzheimer’s disease. Recent studies show that α-KGDH is a target of oxidative stress; reactive oxygen species (ROS) inhibit KGDH activity which diminishes its critical function and can cause a bioenergetic deficit. BioVision’s α-KGDH assay kit provides a quick and easy way for monitoring α-KGDH activity in various samples. In the assay, α-KGDH converts α-ketoglutarate into an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect α-ketoglutarate dehydrogenase activity lower than 0.1 mU in a variety of samples.
• Data Sheet Link
• Additional description
  The Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit is a α- or alpha protein sometimes glycoprotein present in blood.
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Alpha-Ketoglutarate   Dehydrogenase   Activity   Kit  
• Short name
  Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  a-Ketoglutarate Dehydrogenase Activity colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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