Malate Dehydrogenase Activity Colorimetric Assay Kit

• Catalog number
  K654-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly-Sensitive Assay
• Summary
  • Detection method- Absorbance (450 nm) • Applications- - Measurement of malate dehydrogenase activity in various tissues/cells - Analysis of citric acid cycle and malate-asparate shuttle
• Detection Method
  Absorbance (450 nm)
• Applications
  • Measurement of malate dehydrogenase activity in various tissues/cells • Analysis of citric acid cycle and malate-asparate shuttle
• Sample Type
  • Animal tissues such as liver, heart, muscle, etc. • Cell culture: Adherent or suspension cells • Mitochondria
• Features Benefits
  • Simple, rapid & convenient • The assay can detect Malate Dehydrogenase (MDH) Activity less than 0.5 mU in various sample types.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Malate Dehydrogenase (MDH) (EC 1.1.1.37) is an important enzyme which reversibly converts L-malate into oxaloacetate in the presence of NAD. In eukaryotic cells, malate dehydrogenase has 2 isoforms: MDH1 and MDH2. MDH1 is cytosolic & participates in the malate-aspartate shuttle, which transports malate into mitochondria for utilization in ATP generation whereas MDH2 is a mitochondrial enzyme and part of the citric acid cycle. MDH activity is increased in some neurodegenerative diseases such as Alzheimer’s disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma). In BioVision’s Malate Dehydrogenase Activity Assay kit, MDH reacts with malate to form an intermediate. The generated intermediate reacts with MDH Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect less than 0.5 mU of MDH activity in various sample types.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Malate   Dehydrogenase   Activity   Kit  
• Short name
  Malate Dehydrogenase Activity Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Malate Dehydrogenase Activity colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products