Aldehyde Dehydrogenase Activity Colorimetric Assay Kit

• Catalog number
  K731-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Absorbance (450 nm) • Species reactivity- Mammalian • Application- The assay can detect < 0.5 mU of ALDH activity (based on our unit definition) in a variety of samples.
• Sample Type
  Cell and tissue culture supernatants, cell lysates, plasma and serum, as well as many other biological fluids etc.
• Features Benefits
  • Simple procedure • Fast and convenient • Sensitive assay for measuring ALDH activity in various biological samples and the kit is also suitable for high throughput analyses.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis. Therefore the early detection of ALDH activity levels can be prognostic and guide the therapeutic strategies. The BioVision Aldehyde Dehydrogenase (ALDH) Activity Assay Kit is a simple, fast and reliable method to quantify the ALDH enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH generating NADH which then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay can detect < 0.5 mU of ALDH activity (based on our unit definition) in a variety of samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Aldehyde   Dehydrogenase   Activity   Kit  
• Short name
  Aldehyde Dehydrogenase Activity Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Aldehyde Dehydrogenase Activity colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products