6-Phosphogluconate Dehydrogenase Activity Colorimetric Assay Kit

• Catalog number
  K540-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Colorimetric (OD 460 nm) • Applications- Measurement of 6-PGD activity in purified/crude enzyme preparations and biological samples
• Detection Method
  Colorimetric (OD 460 nm)
• Species Reactivity
  Mammalian
• Applications
  Measurement of 6-PGD activity in purified/crude enzyme preparations and biological samples
• Sample Type
  Purified enzyme, tissue and cell lysates
• Features Benefits
  • Simple, rapid & convenient assay to measure 6-PGD Activity in biological samples • Includes Positive Control
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  6-Phosphogluconate dehydrogenase (6-PGD, EC 1.1.1.44) is an enzyme in the pentose phosphate pathway that catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose-5-phosphate with simultaneous reduction of NADP+ to NADPH. Ribulose-5-phosphate is used for production of nucleotides and nucleic acids and can also be reversibly converted to glyceraldehyde-3-phosphate and fructose-6-phosphate which can be used in the glycolysis pathway. It also has a vital role in production of NADPH, which is utilized as a reducing agent in numerous biosynthetic pathways and is important in prevention of oxidative damage. In humans, 6-PGD deficiency is an autosomal hereditary disorder that can result in depletion of NADPH in red blood cells. As NADPH is required for regeneration of the cellular antioxidant glutathione in red blood cells, 6-PGD deficiency increases the risk of hemolytic anemia in situations of oxidative stress. On the other hand, inhibition of 6-PGD has been linked to anti-cancer activity by decreasing RNA biosynthesis and increasing the buildup of reactive oxygen species in tumor cells. BioVision’s 6-PGD assay kit provides a quick, sensitive and easy way for measuring 6-PGD activity in various biological samples. In this assay, 6-PGD converts 6-phosphogluconate into an intermediate, generating NADPH, which subsequently reduces a colorless probe into a strongly colored product detectable by absorbance at 460 nm. The assay is high-throughput adaptable and can detect less than 0.05 mU of 6-PGD activity
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  6-Phosphogluconate   Dehydrogenase   Activity   Kit  
• Short name
  6-Phosphogluconate Dehydrogenase Activity Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  6-Phosphogluconate Dehydrogenase Activity colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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