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Clone
6H9-B6
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Immunogen
Full length protein, BC001304.1
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Antibody s full description
Mouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa Antibody, Clone: 6H9-B6: ATTO 488
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
ACZ Antibody, Aczonin Antibody, PCLO Antibody, Pico Antibody, presynaptic cytomatrix protein Antibody
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Raised in
Mouse
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Antibody s target
Piccolo
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Primary research fields
Neuroscience, Cell Structure, Pre-Synaptic Markers
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Brandname
None
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Antibodies applications
WB, ICC/IF, ELISA
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~550kDa. No cross-reactivity to Bassoon.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-188 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Junction , Synapse
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
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Bibliography
1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.
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Released date
4-Nov-2010
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_001104266.1
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Gene number
26875
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Protein number
Q9QYX7
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 (SMC-188). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody (SMC-188) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Piccolo Antibody (D) Composite. Western Blot analysis of Rat and mouse brain lysates showing detection of Piccolo protein using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 (SMC-188). Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody (SMC-188) at 1:1000. (1) Mouse brain lysate; (2) Rat brain lysate. Mouse Anti-Piccolo Antibody [6H9-B6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-188) Mouse Anti-Piccolo Antibody [6H9-B6] used in Western Blot (WB) on Rat and mouse brain lysates (SMC-188)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus