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Mouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa, Clone 6H9-B6

  • Catalog number
    SMC-188D-A700
  • Product name
    Mouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa, Clone 6H9-B6
  • Size
    100 µg
  • Label
    ATTO 700
  • Clone
    6H9-B6
  • Immunogen
    Full length protein, BC001304.1
  • Antibody s full description
    Mouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa Antibody, Clone: 6H9-B6: ATTO 700
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    ACZ Antibody, Aczonin Antibody, PCLO Antibody, Pico Antibody, presynaptic cytomatrix protein Antibody
  • Raised in
    Mouse
  • Isotype
    IgG2b Kappa, IgG2b
  • Antibody s target
    Piccolo
  • Primary research fields
    Neuroscience, Cell Structure, Pre-Synaptic Markers
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF, ELISA
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~550kDa. No cross-reactivity to Bassoon.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-188 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Junction , Synapse
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
  • Bibliography
    1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.
  • Released date
    4-Nov-2010
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_001104266.1
  • Gene number
    26875
  • Protein number
    Q9QYX7
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 (SMC-188). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody (SMC-188) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Piccolo Antibody (D) Composite. Western Blot analysis of Rat and mouse brain lysates showing detection of Piccolo protein using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 (SMC-188). Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody (SMC-188) at 1:1000. (1) Mouse brain lysate; (2) Rat brain lysate. Mouse Anti-Piccolo Antibody [6H9-B6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-188) Mouse Anti-Piccolo Antibody [6H9-B6] used in Western Blot (WB) on Rat and mouse brain lysates (SMC-188)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    Piccolo Monoclonal IgG2b Kappa 6H9-B6
    • Gene info
    Gene info
    Locus Specific Databases:Global Variome shared LOVD
  • Gene symbol
    PCDHGB6, MAGEB6
  • Host
    mouse
  • isotype filter
    • IgG2b Kappa
  • Short name
    Mouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa, Clone 6H9-B6
  • label filter
    • ATTO 700
  • Clone name
    6H9-B6
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies