Mouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa, Clone 6H9-B6
#
-
Catalog numberSMC-188D-BI
-
Price:
-
Size100 µg
-
-
Clone6H9-B6
-
ImmunogenFull length protein, BC001304.1
-
Antibody s full descriptionMouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa Antibody, Clone: 6H9-B6: Biotin
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other nameACZ Antibody, Aczonin Antibody, PCLO Antibody, Pico Antibody, presynaptic cytomatrix protein Antibody
-
Raised inMouse
-
Antibody s targetPiccolo
-
Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers
-
BrandnameNone
-
Antibodies applicationsWB, ICC/IF, ELISA
-
Antibody s reactivityHuman, Mouse, Rat
-
Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityDetects ~550kDa. No cross-reactivity to Bassoon.
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysis1 µg/ml of SMC-188 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.
-
Antibody in cellCell Junction , Synapse
-
Tissue specificitySee included datasheet or contact our support service
-
Scientific contextPiccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
-
Bibliography1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.
-
Released date4-Nov-2010
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberNP_001104266.1
-
Gene number26875
-
Protein numberQ9QYX7
-
Antibody s datasheetContact our support service
-
Representative figure link
-
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 (SMC-188). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody (SMC-188) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Piccolo Antibody (D) Composite. Western Blot analysis of Rat and mouse brain lysates showing detection of Piccolo protein using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6 (SMC-188). Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody (SMC-188) at 1:1000. (1) Mouse brain lysate; (2) Rat brain lysate. Mouse Anti-Piccolo Antibody [6H9-B6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-188) Mouse Anti-Piccolo Antibody [6H9-B6] used in Western Blot (WB) on Rat and mouse brain lysates (SMC-188)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
Gene target
-
Gene symbolPCDHGB6, MAGEB6
-
Short nameMouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa, Clone 6H9-B6
-
Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse
-
IsotypeIgG2b Kappa, IgG2b
-
LabelBiotin
-
SpeciesMouse, Mouses
-
Alternative nameMouse Antibody toMouse Piccolo monoclonal IgG2b Kappa, clonality 6H9-B6
-
Alternative techniquemurine, antibodies
-
Clone name6H9-B6
-
Gene info
-
Identity
-
Gene
-
Long gene nameprotocadherin gamma subfamily B, 6
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-06-28
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Clustered protocadherins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameMAGE family member B6
-
Synonyms gene name
- melanoma antigen family B6
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2003-12-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- MAGE family
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data