Rabbit IgG, conjugated with FITC, Isotype control

• Catalog number
  RIgG-FITC
• Price
  Please ask
• Size
  1ml

• Host Source
  NA
• Clone Name
  NA
• Product Category
  Veterinary
• Species Reactivity
  Rabbit
• Application
  ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
• Immunogen
  NA
• Purification Method
  NA
• Shipping Conditions
  NA
• Storage conditions
  Rabbit IgG, conjugated with FITC, Isotype control lized at +4ÁC--at least 10 years. Reconstituted at or below -20ÁC--3-5 years. Reconstituted at +4ÁC--7 days.
• Datasheet link
  NA
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples.
• Properties
  This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by Exalpha. Rabbit antibodies are very stable and can be stored for several days at room temperature. Exalpha adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Group
  isotypes
• Latin name
  Oryctolagus cuniculus

• Gene target
  conjugated   with  
• Short name
  Rabbit IgG, conjugated with FITC, Isotype control
• Technique
  Control, Isotype, Rabbit, FITC, IgG, antibody Conjugates, controls, Fluorescein, IgGs, Isotypes and controls
• Host
  Rabbit, Rabbits
• Isotype
  NA, IgG
• Label
  FITC
• Alternative name
  production species: rabbit Immunoglobulin G, coupled including fluorecein, isoform reference
• Alternative technique
  control, controls, rabbit-anti, fluorescine, immunoglobulins
• Tissue
  control

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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