Rabbit IgG Isotype Control- FITC Conjugated

• Catalog number
  MBS669059
• Price
  Please ask
• Size
  5x0.1 mg

• Products_type
  Isotype Control
• Products_short_name
  [IgG]
• Products_gene_name
  [IgG]
• Clonality
  Polyclonal
• Purity
  Purified Affinity Chromatography
• Form
  0.2 mg/ml in Tris buffer containing 0.05% Azide
• Storage_stability
  Store the antibody at 4 degree C; stable for 6 months.
• Tested_application
  ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Group
  isotypes
• Latin name
  Oryctolagus cuniculus

• Gene target
  Conjugated  
• Short name
  Rabbit IgG Isotype Control- FITC Conjugated
• Technique
  Control, Isotype, Rabbit, FITC, IgG, antibody Conjugates, controls, Fluorescein, IgGs, Isotypes and controls
• Host
  Rabbit, Rabbits
• Isotype
  IgG, IgG
• Label
  FITC
• Alternative name
  production species: rabbit Immunoglobulin G isoform reference- fluorecein coupled
• Alternative technique
  control, controls, rabbit-anti, fluorescine, immunoglobulins
• Tissue
  control

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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