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Immunogen
A synthesized peptide derived from human p21 Cip1
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Antibody s target
Human p21
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Antibody s full description
Rabbit Anti-Human p21 Polyclonal
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Primary research fields
Cancer, Cell Cycle, Cip/Kip, Cell Signaling, Epigenetics and Nuclear Signalling
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
CDKN2A Antibody, CDKN2 Antibody, MTS1 Antibody, Cyclin-dependent kinase inhibitor 2A Antibody, isoforms 1/2/3, Cyclin-dependent kinase 4 inhibitor A Antibody, CDK4I Antibody, p16-INK4a Antibody, Multiple tumor suppressor 1 Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:2000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~21kDa, endogenous levels of total p21 Cip1
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1281 was sufficient for detection of p21 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
Expressed in all adult human tissues
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Scientific context
p21 is a potent cyclin-dependent kinase inhibitor(Cdks). It is a novel Cdk-interacting protein in that it has been identified in cyclin A, cyclin D1, cyclin E and Cdk2 immunoprecipitates. p21 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk 2, cyclin E-Cdk2, cyclin D1-Cdk4 and cyclin D2-Cdk4 complexes. Expression of p21 is inducible by wildtype, but not mutant, p53.
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Bibliography
1. "Entrez Gene: CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1)". 2. Harper J.W., Adami G.R., Wei N., Keyomarsi K., Elledge S.J. (1993) Cell. 75(4): 805–816. 3. el-Deiry W.S., et al. (1993). Cell. 75(4): 817–825. 4. Xiong Y., et al. (1993) Nature. 366: 701–704. 5. Rodriguez R., Meuth M. (2006) Mol. Biol. Cell. 17(1): 402–412.
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Released date
6/Sep/2016
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NCBI number
NP_000380.1
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Gene number
1026
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Protein number
P38936
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human HeLa cell lysates showing detection of ~26kDa p21 protein using Rabbit Anti-p21 Polyclonal Antibody (SPC-1281). Lane 1: EGF treated Human HeLa whole cell lysates treated with the immunizing peptide. Lane 2: EGF treated Human HeLa whole cell lysates. Primary Antibody: Rabbit Anti-p21 Polyclonal Antibody (SPC-1281) at 1:1000. Predicted/Observed Size: ~26kDa. Rabbit Anti-p21 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1281)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
