Rabbit Anti-Human p53 Polyclona: ATTO 390
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Catalog numberSPC-682D-A390
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Price:
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Size100 µg
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ImmunogenSynthetic peptide from the C-terminal of human tumor protein p53.
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Antibody s targetHuman p53
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Antibody s full descriptionRabbit Anti-Human p53 Polyclona
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Primary research fieldsCancer, Cell Cycle, p53 Pathway, Cancer, Tumor suppressors, Apoptosis, Cell Signaling, Epigenetics and Nuclear Signalling, DNA/RNA, DNA Damage and Repair
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameTP53 Antibody, Tumor Protein 53 Antibody, BCC7 Antibody, LFS1 Antibody, TRP53 Antibody, p53 tumor suppressor Antibody
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Verified applicationsWB, ICC/IF
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Raised inRabbit
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Antibody s reactivityHuman
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Antibody s recommended dilutions for useWB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityPredicted molecular weight at ~43.7kDa. Observed bands at ~53kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-682 was sufficient for detection of p53 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm, Nucleus, PML body, Endoplasmic Reticulum, Mitochondrion matrix
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextThe p53 protein (tumor protein 53 or TP53) is a DNA-binding cell cycle-regulating transcription factor that governs cell division and the fine balance between cell death and cell survival (1). P53 plays a critical role in tumor suppression and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman." This also refers to its role in conserving stability by preventing genome mutation (2). Defects in p53 are linked to >50% of human cancers, and restoring p53 function to these cancer cells can induce growth arrest and apoptosis (3). When p53 has been damaged, it can also lead to autoimmune disorders (4, 5).
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Bibliography1. Georescu C.V., Saftoiu A., Georgescu C.C., Ciurea R. and Ciurea T. (2007) J Gastrointestin Liver Dis. 16(2): 133-9. 2. Strachan T., et al. (1999) Human Molecular Genetics 2. Ch. 18: Cancer Genetics. 3. Hansen J.E., et al. (2007) Cancer Research 67: 1769-1774. 4. Di Cesare E., et al. (2001) Ann Clin Lab Sci 31: 253-8. 5. Akere A., Otegbayo J.A. (2007) Singapore Med J. 48(1): 41-4.
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Released date1/Oct/2015
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NCBI numberNP_000537.3
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Gene number7157
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Protein numberP04637
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p53 Polyclonal Antibody (SPC-682). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody (SPC-682) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, PML body, Endoplasmic Reticulum. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) p53 Antibody (D) Composite. | Western blot analysis of Human HeLa and 293T cell lysates showing detection of ~43.7kDa p53 protein using Rabbit Anti-p53 Polyclonal Antibody (SPC-682). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody (SPC-682) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~43.7kDa. | Western blot analysis of Human A431 showing detection of ~43.7kDa p53 protein using Rabbit Anti-p53 Polyclonal Antibody (SPC-682). Lane 1: MW Ladder. Lane 2: Human A431 (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody (SPC-682) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~43.7kDa. Rabbit Anti-p53 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cervical Cancer cell line (HeLa) (SPC-682) | Rabbit Anti-p53 Antibody used in Western blot (WB) on HeLa and 293T cell lysates (SPC-682) | Rabbit Anti-p53 Antibody used in Western blot (WB) on A431 (SPC-682)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutRabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Short nameRabbit Anti- p53 Polyclona: ATTO 390
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Techniqueanti-Human, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 390
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman p53 Polyclona: ATTO 390
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Alternative techniquerabbit-anti, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data