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Immunogen
Human native full-length BVR
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Antibody s target
Human BVR
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Antibody s full description
Rabbit Anti-Human BVR Polyclonal
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Primary research fields
Cancer, Oxidative Stress, Cell Signaling
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Biliverdin Reductase Antibody, BIEA_HUMAN Antibody, Biliverdin IX alpha reductase Antibody, Biliverdin reductase A Antibody, Biliverdin-IX alpha-reductase Antibody, BLVR A Antibody, BLVR Antibody, Blvra Antibody, BVR A Antibody, BVRA Antibody, Zinc metalloprotein Antibody, zinc-metalloprotein Antibody
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Verified applications
WB, IHC, ICC/IF, IP
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:2000), ICC/IF (1:120), IP (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~40-42kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
1 µg/ml of SPC-214 was sufficient for detection of BVR in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Biliverdin Reductase (BVR) is a cytoplasmic enzyme that catalyzes the conversion of biliverdin to bilirubin by converting a double bond between the second and third pyrrole ring into a single bond (1). It is ubiqutiously expressed in all tissues- it occurs in cells and brain regiuons that already display HO-1 and HO-2, but also in regions and cell types with potential to induce stress proteins. It is unique among all enzymes in having two pH optima, using a different cofactor at each pH range, NADH at pH7.0 and NADPH at pH8.7 (2). It is not inactivated by heat shock, and have shown to abate inflammation, oxidative stress and apoptosis (3).
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Bibliography
1. Singleton J.W., Laster L. (1965). J Biol Chem. 240: 4780-4789. 2. Kutty R.K., Maines M.D. (1981) J Biol Chem. 256: 3956-3962. 3. Mishra M., Ndisand J.F. (2014) Curr Pharm Des. 20(9): 1370-1391.
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Released date
7/May/2014
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NCBI number
NP_000703.2
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Gene number
644
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Protein number
P53004
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BVR Polyclonal Antibody (SPC-214). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-BVR Polyclonal Antibody (SPC-214) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Exosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-BVR Antibody. (C) Composite. | Western blot analysis of Human, Rat Brain cell lysates showing detection of BVR protein using Rabbit Anti-BVR Polyclonal Antibody (SPC-214). Lane 1: Rat Brain. Lane 2: Human Brain lysates. Load: 10 µg. Primary Antibody: Rabbit Anti-BVR Polyclonal Antibody (SPC-214) at 1:1000. Rabbit Anti-BVR Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-214) | Rabbit Anti-BVR Antibody used in Western blot (WB) on Human, Rat Brain cell lysates (SPC-214)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
