-
Immunogen
Synthesized peptide derived from human Collagen IV.
-
Antibody s target
Human Collagen IV
-
Antibody s full description
Rabbit Anti-Human Collagen IV Polyclonal
-
Primary research fields
Stem Cells, Mesenchymal Stem Cells, Chondrogenesis, Cell Signaling, Extracellular Matrix (ECM), Collagen, Cancer, Invasion/microenvironment
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
Arresten Antibody, CO4A1_HUMAN Antibody, collagen alpha-1(IV) chain Antibody, Collagen Of Basement Membrane Alpha 1 Chain Antibody, Collagen Type IV Antibody
-
Verified applications
WB, IHC, ELISA
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse
-
Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
-
Antibody s concentration
See included datasheet or contact our support service
-
Antibody s specificity
Detects endogenous levels of total Collagen IV protein.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:1000 dilution of SPC-1276 was sufficient for detection of Collagen IV in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Extracellular matrix
-
Tissue specificity
Highly expressed in placenta.
-
Scientific context
Type-IV collagen is found primarily in the basal lamina. It has been associated with Goodpasture's Disease, as well as Alport Syndrome.
-
Bibliography
1. Turner N., et al. (1992) J Clin Invest. 89(2): 592–601. 2. "Entrez Gene: COL4A3 collagen, type IV, alpha 3 (Goodpasture antigen)".
-
Released date
6/Sep/2016
-
NCBI number
NP_001836.3
-
Gene number
1282
-
Protein number
P02462
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
Representative figure link
-
Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human HeLa cell lysates showing detection of ~160kDa Collagen IV protein using Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276). Lane 1: Human HeLa cells. Lane 2: Human HeLa cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276) at 1:1000. Predicted/Observed Size: ~160kDa. Rabbit Anti-Collagen IV Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1276) | Rabbit Anti-Collagen IV Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1276)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Gene
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
