Rabbit Anti-Human FNIP1 Polyclonal: ATTO 594
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Catalog numberSPC-718D-A594
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Price:
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Size100 µg
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ImmunogenSynthetic peptide from the mid-protein of Human FNIP1
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Antibody s targetHuman FNIP1
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Antibody s full descriptionRabbit Anti-Human FNIP1 Polyclonal
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Primary research fieldsCancer, Cell Cycle, mTOR Pathway, Cancer, Autophagy
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameFNIP1 Antibody, Folliculin-interacting protein 1 Antibody, Folliculin interacting protein 1 Antibody, KIAA1961 Antibody
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Verified applicationsWB, ICC/IF
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Raised inRabbit
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Antibody s reactivityMouse, Rat
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Antibody s recommended dilutions for useWB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~130 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-718 was sufficient for detection of FNIP1 in 15 µg of mouse kidney cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm
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Tissue specificityStrong expression is found in the heart, liver placenta, muscle, nasal mucosa, salivary gland and uvula and moderate expression in kidney and lung. Higher levels detected in clear cell renal cell carcinoma (RCC) and chromophobe RCC than in normal kidney tissue.
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Scientific contextSee included datasheet or contact our support service.
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BibliographyRefer to PubMed
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Released date25/Oct/2016
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NCBI numberNP_001008738.2
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Gene number96459
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Protein numberQ8TF40
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-FNIP1 Polyclonal Antibody (SPC-718). Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody (SPC-718) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FNIP1 Antibody (D) Composite. | Western blot analysis of Mouse, Rat Kidney showing detection of ~131 kDa FNIP1 protein using Rabbit Anti-FNIP1 Polyclonal Antibody (SPC-718). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Lane 3: Rat Kidney cell lysates. Load: 20 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody (SPC-718) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~131 kDa. Rabbit Anti-FNIP1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on C2C12 Cells (Mouse Myoblast cell line) (SPC-718) | Rabbit Anti-FNIP1 Antibody used in Western blot (WB) on Kidney (SPC-718)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolFNIP1
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Short nameRabbit Anti- FNIP1 Polyclonal: ATTO 594
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 594
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman FNIP1 polyclonal: ATTO 594
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene namefolliculin interacting protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-08-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DENN domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data