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Immunogen
Synthetic peptide from the mid-protein of Human ATG3
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Antibody s target
Human ATG3
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Antibody s full description
Rabbit Anti-Human ATG3 Polyclonal
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Primary research fields
Neuroscience, Neurodegeneration, Cancer, Tumor suppressors, Cardiovascular System, Heart, Cancer, Autophagy, Cell Signaling, Metabolism, Metabolism processes
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ATG3 Antibody, ATG3 autophagy related 3 homolog (S. cerevisiae) Antibody, Ubiquitin-like-conjugating enzyme ATG3 Antibody, APG3-like Antibody, APG3L Antibody, APG3, S. cerevisiae, homolog of Antibody, APG3 like Antibody, Apg 3 Antibody, Autophagy-related protein 3 Antibody, Apg3p Antibody, APG3 Antibody, ATG 3 Antibody, hApg3 Antibody, Autophagy related protein 3 Antibody, Autophagy 3, S. cerevisiae, homolog of Antibody, autophagy related 3 Antibody, ATG3_HUMAN Antibody, Protein PC3-96 Antibody, Autophagy Apg3p/Aut1p like Antibody, PC3 96 Antibody, APG3 autophagy 3 like Antibody, ATG3 autophagy related 3 homolog Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~35 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-671 was sufficient for detection of ATG3 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
25/Oct/2016
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NCBI number
NP_001265641.1
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Gene number
64422
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Protein number
Q9NT62
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human HeLa cell lysates showing detection of ~35.9 kDa ATG3 protein using Rabbit Anti-ATG3 Polyclonal Antibody (SPC-671). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody (SPC-671) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~35.9 kDa. Rabbit Anti-ATG3 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-671)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
ATG3
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Short name
Rabbit Anti- ATG3 Polyclonal: ATTO 633
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
ATTO 633
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman autophagy related 3 polyclonal: ATTO 633
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
autophagy related 3, ATG3 and IDBG-49891 and ENSG00000144848 and 64422, enzyme binding, Cytoplasm, Atg3 and IDBG-162821 and ENSMUSG00000022663 and 67841, ATG3 and IDBG-632300 and ENSBTAG00000009084 and 508571
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