Rabbit Anti-Human Kir4.1 Polyclonal: ATTO 633
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Catalog numberSPC-700D-A633
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Price:
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Size100 µg
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ImmunogenSynthetic peptide from the mid-protein of Human Kir4.1
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Antibody s targetHuman Kir4.1
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Antibody s full descriptionRabbit Anti-Human Kir4.1 Polyclonal
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Primary research fieldsNeuroscience, Ion Channels, Potassium Channels, Inward-Rectifying Potassium Channels
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameATP sensitive inward rectifier potassium channel 10 Antibody, BIRK10 Antibody, KCNJ13 PEN Antibody, Potassium channel inwardly rectifying subfamily J member 10 Antibody, SESAME Antibody inwardly rectifying subfamily J member 10 Antibody, Inward rectifier K(+) channel Kir1.2 Antibody, Potassium channel KCNJ 10 Antibody, ATP-sensitive inward rectifier potassium channel 10 Antibody, Potassium inwardly rectifying channel subfamily J member 10 Antibody, KIR1.2 Antibody, Inwardly rectifying potassium channel Kir1.2 Antibody, ATP-dependent inwardly rectifying potassium channel Kir4.1 Antibody, Glial ATP dependent inwardly rectifying potassium channel KIR4.1 Antibody, Kcnj10 Antibody, KCNJ10 Antibody, IRK10_HUMAN Antibody, KIR4.1 Antibody, Inward rectifier K+ channel KIR1.2 Antibody, ATP dependent inwardly rectifying potassium channel Kir4.1 Antibody, Potassium channel Antibody
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Verified applicationsWB
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Raised inRabbit
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Antibody s reactivityRat
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Antibody s recommended dilutions for useWB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~42 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-700 was sufficient for detection of Kir4.1 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellMembrane, Multi-Pass Membrane Protein, Basolateral Cell Membrane
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Tissue specificityExpressed in kidney (at protein level).
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Scientific contextSee included datasheet or contact our support service.
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BibliographyRefer to PubMed
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Released date25/Oct/2016
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NCBI numberNP_002232.2
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Gene number3766
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Protein numberP78508
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure legendWestern blot analysis of Rat Liver cell lysates showing detection of ~42.5 kDa Kir4.1 protein using Rabbit Anti-Kir4.1 Polyclonal Antibody (SPC-700). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Kir4.1 Polyclonal Antibody (SPC-700) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42.5 kDa. Rabbit Anti-Kir4.1 Antibody used in Western blot (WB) on Liver cell lysates (SPC-700)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Short nameRabbit Anti- Kir4 1 Polyclonal: ATTO 633
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 633
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman Kir4.1 polyclonal: ATTO 633
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data