Rabbit Anti-Human ARAF Polyclonal: ATTO 633
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Catalog numberSPC-1103D-A633
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Price:
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Size100 µl
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ImmunogenSynthetic peptide of human ARaf (AA11-25)
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Antibody s targetHuman ARAF
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Antibody s full descriptionRabbit Anti-Human ARAF Polyclonal
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameA raf 1 antibody, A Raf proto oncogene serine/threonine protein kinase antibody, ARAF 1 antibody, Araf antibody, ARaf proto oncogene serine/threonine protein kinase antibody, ARAF_HUMAN antibody, ARAF1 antibody, PKS 2 antibody, PKS antibody, PKS2 antibody, RAFA 1 antibody, RAFA1 antibody, Ras binding protein DA Raf antibody, Serine/threonine-protein kinase A-Raf antibody, v raf murine sarcoma 3611 viral oncogene homolog antibody
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Verified applicationsWB, AM
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Raised inRabbit
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Antibody s reactivityHuman, Mouse
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Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects 68 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:250 dilution of SPC-1103 was sufficient for detection of ARAF in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytosol
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Tissue specificityPredominantly in urogenital tissues.
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Scientific contextARAF is a tyrosine kinase-like kinase of the Raf family. It is involved in the transduction of mitogenic signals from the cell membrane receptors via Ras and MEK1/2 to the nucleus. It has been implicated in playing a role in the postsynaptic responses of hippocampal neuron. It is inhibited by Nexavar/Sorafenib (IC50>50 nM).
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BibliographyRefer to PubMed
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Released date29/Aug/2016
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NCBI numberNP_001645
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Gene number369
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Protein numberP10398
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolARAF
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Short nameRabbit Anti- ARAF Polyclonal: ATTO 633
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 633
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman ARAF polyclonal: ATTO 633
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameA-Raf proto-oncogene, serine/threonine kinase
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Synonyms gene
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Synonyms gene name
- v-raf murine sarcoma 3611 viral oncogene homolog 1
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Classification
- Mitogen-activated protein kinase kinase kinases
- RAF family
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data