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Immunogen
Synthetic peptide of human Akt2 (AA454-468)
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Antibody s target
Human AKT2
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Antibody s full description
Rabbit Anti-Human AKT2 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Akt2 antibody, AKT2_HUMAN antibody, HIHGHH antibody, PKB antibody, PKB beta antibody, PKBB antibody, PKBBETA antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-BETA antibody, RAC-beta serine/threonine-protein kinase antibody, RAC-PK-beta antibody, v akt murine thymoma viral oncogene homolog 2 antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 55.769 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1101 was sufficient for detection of AKT2 in 10 µg of HEK293 cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus, Cell Membrane
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Tissue specificity
Expressed in all human cell types so far analyzed.
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Scientific context
AKT2 is a protein-serine/threonine kinase. It is closely related to AKT1 and AKT3. Its cell signalling activities involve activating cell surival and proliferation, increasing metabolism, and angiogenesis. Its interaction network contains over 100 substrates which includes inhibiting p53 via downstream signalling, activating mTOR, inhibition of GSK3B, and inhibition of cyclins. In cancer, it is found to be overexpressed and amplified. It is also linked with invasion, cell migration and induction of angiogenesis.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_001617
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Gene number
208
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Protein number
P31751
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
AKT2
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Short name
Rabbit Anti- AKT2 Polyclonal: ATTO 594
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
ATTO 594
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman v-akt murine thymoma viral oncogene homolog 2 polyclonal: ATTO 594
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
v-akt murine thymoma viral oncogene homolog 2, HIHGHH and PKBB and PKBBETA and PRKBB and RAC-BETA, AKT2 and IDBG-51270 and ENSG00000105221 and 208, transferase activity, nuclei, Akt2 and IDBG-161456 and ENSMUSG00000004056 and 11652, AKT2 and IDBG-637808 and ENSBTAG00000001400 and 534923
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