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Rabbit Anti-Human Akt2 (PKB beta) Polyclonal: ATTO 488

  • Catalog number
    SPC-148F-A488
  • Product name
    Rabbit Anti-Human Akt2 (PKB beta) Polyclonal: ATTO 488
  • Size
    400 µl
  • Label
    ATTO 488
  • Immunogen
    A five residue synthetic peptide based on the human Akt2, coupled to KLH
  • Antibody s target
    Human AKT2
  • Antibody s full description
    Rabbit Anti-Human Akt2 (PKB beta) Polyclonal
  • Primary research fields
    Cell Signaling, Post-Translational Modifications, Phosphorylation
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    AKT2 Antibody, PKB Antibody, PKB beta Antibody, PRKBB Antibody, protein kinase Akt2 Antibody, RAC BETA Antibody
  • Verified applications
    WB, ICC/IF, ELISA
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Rabbit, Sheep, African clawed frog (Xenopus laevis)
  • Antibody s recommended dilutions for use
    WB (1:1000), ICC/IF (1:25); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    TBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    0.25 mg/ml
  • Antibody s specificity
    Detects ~65kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3).
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:3000 dilution of SPC-148 was sufficient for detection of PKBβ in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
  • Antibody in cell
    Cytoplasm, Nucleus, Cell Membrane
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
  • Bibliography
    1. Downward J. (1998). Curr Opin Cell Biol. 10(2): 262-7. 2. Brazil D.P., and Hemmings B.A. (2001). Biochem Sci. 26(11):657-64. 3. Nicholson K.M., Anderson N.G. (2002). Cell Signal. 14(5): 381-95. 4. Hardie, G., Hanks, S. (1995). The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps.
  • Released date
    1/Jun/2007
  • NCBI number
    NP_001617.1
  • Gene number
    208
  • Protein number
    P31751
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:25 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AKT2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of multiple Cell line lysates showing detection of AKT2 protein using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. Rabbit Anti-AKT2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-148) | Rabbit Anti-AKT2 Antibody used in Western blot (WB) on multiple Cell line lysates (SPC-148)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.4
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Alternative to gene target
    • [ "v-akt murine thymoma viral oncogene homolog 2", "HIHGHH and PKBB and PKBBETA and PRKBB and RAC-BETA", "AKT2 and IDBG-51270 and ENSG00000105221 and 208", "transferase activity", "nuclei", "Akt2 and IDBG-161456 and ENSMUSG00000004056 and 11652", "AKT2 and IDBG-637808 and ENSBTAG00000001400 and 534923" ]
  • Gene target
    Akt2 PKB beta ATTO 488
    • Gene info
    Locus Specific Databases:LRG_721
  • Gene symbol
    AKT1
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- Akt2 (PKB beta) Polyclonal: ATTO 488
  • label filter
    • ATTO 488
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies