-
Immunogen
A five residue synthetic peptide based on the human Akt2, coupled to KLH
-
Antibody s target
Human AKT2
-
Antibody s full description
Rabbit Anti-Human Akt2 (PKB beta) Polyclonal
-
Primary research fields
Cell Signaling, Post-Translational Modifications, Phosphorylation
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
AKT2 Antibody, PKB Antibody, PKB beta Antibody, PRKBB Antibody, protein kinase Akt2 Antibody, RAC BETA Antibody
-
Verified applications
WB, ICC/IF, ELISA
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Rabbit, Sheep, African clawed frog (Xenopus laevis)
-
Antibody s recommended dilutions for use
WB (1:1000), ICC/IF (1:25); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Protein A Purified
-
Recommended buffer for storage
TBS, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration
0.25 mg/ml
-
Antibody s specificity
Detects ~65kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3).
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:3000 dilution of SPC-148 was sufficient for detection of PKBβ in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
-
Antibody in cell
Cytoplasm, Nucleus, Cell Membrane
-
Tissue specificity
See included datasheet or contact our support service.
-
Scientific context
Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
-
Bibliography
1. Downward J. (1998). Curr Opin Cell Biol. 10(2): 262-7. 2. Brazil D.P., and Hemmings B.A. (2001). Biochem Sci. 26(11):657-64. 3. Nicholson K.M., Anderson N.G. (2002). Cell Signal. 14(5): 381-95. 4. Hardie, G., Hanks, S. (1995). The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps.
-
Released date
1/Jun/2007
-
NCBI number
NP_001617.1
-
Gene number
208
-
Protein number
P31751
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
Representative figure link
-
Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:25 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AKT2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of multiple Cell line lysates showing detection of AKT2 protein using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. Rabbit Anti-AKT2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-148) | Rabbit Anti-AKT2 Antibody used in Western blot (WB) on multiple Cell line lysates (SPC-148)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.4
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Gene target
-
Gene symbol
AKT1
-
Short name
Rabbit Anti- Akt2 (PKB beta) Polyclonal: PE/ATTO 594
-
Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
Immunoglobulin
-
Label
PE/ATTO 594
-
Species
Human, Humans
-
Alternative name
production species: rabbit Antibody toHuman v-akt murine thymoma viral oncogene homolog 2 (PKB b) polyclonal: peroxidase/ATTO 594
-
Alternative technique
polyclonals, rabbit-anti, antibodies
-
Alternative to gene target
v-akt murine thymoma viral oncogene homolog 2, HIHGHH and PKBB and PKBBETA and PRKBB and RAC-BETA, AKT2 and IDBG-51270 and ENSG00000105221 and 208, transferase activity, nuclei, Akt2 and IDBG-161456 and ENSMUSG00000004056 and 11652, AKT2 and IDBG-637808 and ENSBTAG00000001400 and 534923
-
