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Clone
S70-28
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Immunogen
Fusion protein amino acids 778-910 (C terminus) of rat HCN1
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Antibody s full description
Mouse Anti-Rat HCN1 Monoclonal IgG1 Antibody, Clone: S70-28: ATTO 390
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
BCNG-1 Antibody, BCNG1 Antibody, Brain cyclic nucleotide gated channel 1 Antibody, Brain cyclic nucleotide-gated channel 1 Antibody, HAC2 Antibody, HCN1 Antibody, HCN1_HUMAN Antibody, Hyperpolarization activated cyclic nucleotide gated potassium channel 1 Antibody, Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 Antibody, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 Antibody
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Raised in
Mouse
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Antibody s target
HCN1
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Primary research fields
Neuroscience, Ion Channels, Cyclic Nucleotide-Gated Ion Channels, cardiovascular System, Heart
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Brandname
None
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Antibodies applications
WB, IHC, IP, AM
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~100kDa. No cross-reactivity against HCN2.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-304 was sufficient for detection of HCN1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, play a crucial role in the regulatons of cell excitability. Importantly, they contribute to spontaneous rhythmic activity in both the heart and brain (1).
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Bibliography
1. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34232.
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Released date
6-Jul-2009
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_445827.1
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Gene number
84390
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Protein number
Q9JKBO
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (SMC-304). Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (SMC-304) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells. Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (SMC-304). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (SMC-304) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (SMC-304). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (SMC-304) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (SMC-304). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (SMC-304) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons. Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (SMC-304). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (SMC-304) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Mouse Anti-HCN1 Antibody [S70-28] used in Immunohistochemistry (IHC) on Mouse Cerebellum (SMC-304) Mouse Anti-HCN1 Antibody [S70-28] used in Western Blot (WB) on Rat brain membrane lysate (SMC-304) Mouse Anti-HCN1 Antibody [S70-28] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-304) Mouse Anti-HCN1 Antibody [S70-28] used in Immunohistochemistry (IHC) on Mouse Frozen brain section (SMC-304) Mouse Anti-HCN1 Antibody [S70-28] used in Immunohistochemistry (IHC) on Mouse Frozen brain section (SMC-304)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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Gene target
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Gene symbol
IGHV4-28, SNORD114-28, RNVU1-28, SNORD115-28, SNORD116-28, ERVW-28, ERVK-28, IGKV2-28, IGHVII-28-1, IGKV2D-28
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Short name
Mouse Anti- HCN1 Monoclonal IgG1, Clone S70-28
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Technique
anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG1, IgG1
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Label
ATTO 390
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Species
Rat, Mouses, Rats
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Alternative name
Mouse Antibody toRat HCN1 monoclonal IgG1, clonality S70-28
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Alternative technique
murine, antibodies
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Clone name
S70-28
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