Mouse Anti-Rat HCN1 Monoclonal IgG1, Clone S70-28
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Catalog numberSMC-304D-HRP
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PricePlease ask
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Size100 µg
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CloneS70-28
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ImmunogenFusion protein amino acids 778-910 (C terminus) of rat HCN1
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Antibody s full descriptionMouse Anti-Rat HCN1 Monoclonal IgG1 Antibody, Clone: S70-28: HRP
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameBCNG-1 Antibody, BCNG1 Antibody, Brain cyclic nucleotide gated channel 1 Antibody, Brain cyclic nucleotide-gated channel 1 Antibody, HAC2 Antibody, HCN1 Antibody, HCN1_HUMAN Antibody, Hyperpolarization activated cyclic nucleotide gated potassium channel 1 Antibody, Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 Antibody, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 Antibody
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Raised inMouse
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Antibody s targetHCN1
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Primary research fieldsNeuroscience, Ion Channels, Cyclic Nucleotide-Gated Ion Channels, cardiovascular System, Heart
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BrandnameNone
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Antibodies applicationsWB, IHC, IP, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~100kDa. No cross-reactivity against HCN2.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-304 was sufficient for detection of HCN1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextHyperpolarization-activated cation channels of the HCN gene family, such as HCN1, play a crucial role in the regulatons of cell excitability. Importantly, they contribute to spontaneous rhythmic activity in both the heart and brain (1).
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Bibliography1. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34232.
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Released date6-Jul-2009
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_445827.1
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Gene number84390
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Protein numberQ9JKBO
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (SMC-304). Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (SMC-304) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells. Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (SMC-304). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (SMC-304) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (SMC-304). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (SMC-304) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (SMC-304). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (SMC-304) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons. Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (SMC-304). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (SMC-304) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Mouse Anti-HCN1 Antibody [S70-28] used in Immunohistochemistry (IHC) on Mouse Cerebellum (SMC-304) Mouse Anti-HCN1 Antibody [S70-28] used in Western Blot (WB) on Rat brain membrane lysate (SMC-304) Mouse Anti-HCN1 Antibody [S70-28] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-304) Mouse Anti-HCN1 Antibody [S70-28] used in Immunohistochemistry (IHC) on Mouse Frozen brain section (SMC-304) Mouse Anti-HCN1 Antibody [S70-28] used in Immunohistochemistry (IHC) on Mouse Frozen brain section (SMC-304)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolIGHV4-28, SNORD114-28, RNVU1-28, SNORD116-28, SNORD115-28, ERVW-28, ERVK-28, IGKV2-28, IGHVII-28-1, IGLV2-28
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Short nameMouse Anti- HCN1 Monoclonal IgG1, Clone S70-28
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelHRP
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat HCN1 monoclonal IgG1, clonality S70-28
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Alternative techniquemurine, antibodies
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Clone nameS70-28
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 4-28
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-28
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 28
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Locus
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Discovery year2019-07-26
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Classification
- Variant U1 small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-28
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-28
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Synonyms
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Locus
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Discovery year2011-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group W member 28
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Synonyms gene name
- endogenous retrovirus group W, member 28
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GenBank acession
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Locus
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Discovery year2013-10-21
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 28
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Synonyms gene name
- endogenous retrovirus group K, member 28
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GenBank acession
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Locus
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Discovery year2014-01-03
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-28
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-28-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-28-1
- immunoglobulin heavy variable (II)-28-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 2-28 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable 2-28
- immunoglobulin lambda variable 2-28 pseudogene
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data