Rat IgG1 Mouse Isotype Control Monoclonal Antibody [RTK2071]-FITC

  • Catalog number
    BD-RICIGG1-02MG
  • Price
    Please ask
  • Size
    0,2 mg
  • Description
    Rat IgG1 Mouse Isotype Control Monoclonal Antibody [RTK2071]-FITC is a Rat IgG1 monoclonal antibody type mouse isotype control, produced by [RTK2071] clones, this reagent was conjugated with FITC format and reported for use in flow cytometric analysis as a negative control, it's used for testing the reliabilty of biological tests. Use the same concetration of the specific antibody of interest.
  • Clone
    RTK2071
  • Conjugate
    FITC
  • Application
    Flow Cytometry Method
  • Formulation
    Phosphate buffred solution, 0,09% sodium Azide, may contain carrier protein-stabilizer
  • Expiration
    After 1 year
  • Storage
    2-8°C, store in dark, do not freeze
  • Remark
    For Recherche Use Only.
  • Properties
    If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Additional description
    Isotype or positive controls by peptides, antibodies and deactivated samples.
  • Conjugation
    Anti-FITC Antibody
  • Group
    isotypes
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • French translation
    anticorps
  • Gene target
  • Short name
    IgG1 Mouse Isotype Control Monoclonal Antibody [RTK2071]-FITC
  • Technique
    Antibody, Control, Isotype, Mouse, FITC, antibodies against human proteins, antibodies for, controls, Fluorescein, Isotypes and controls, Monoclonals or monoclonal antibodies, mouses
  • Host
    Rat
  • Isotype
    IgG1, IgG1
  • Label
    FITC
  • Species
    Mouse, Mouses, Rats
  • Alternative name
    Anti Rat IgG1 Mouse Isotype Mouse Control-FITC
  • Alternative technique
    antibodies, control, controls, murine, fluorescine
  • Tissue
    control
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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