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Clone
S2-16
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Immunogen
Fusion protein amino acids 1052-1121 of mouse Slo3
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Antibody s full description
Mouse Anti-Mouse Slo3 Monoclonal IgG2b Antibody, Clone: S2-16: Biotin
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Kcnma3 Antibody, mSlo3 Antibody, Slo3 Antibody, KCa5 Antibody, KCNMA3 Antibody, potassium channel subfamily U member 1 Antibody, Slowpoke homolog 3 Antibody, KCa5.1 Antibody, KCNMC1Calcium-activated potassium channel Antibody, subfamily M subunit alpha-3 Antibody, Calcium-activated potassium channel subunit alpha-3 Antibody, potassium channel Antibody, subfamily U Antibody, member 1 Antibody
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Raised in
Mouse
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Antibody s target
Slo3
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Primary research fields
Neuroscience, Ion Channels, Potassium Channels, Calcium-Activated Potassium Channels
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF , AM
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~115kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-326 was sufficient for detection of Slo3 in 10 µg of mouse brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
The Slo3 channel is a novel potassium channel abundantly expressed in mammalian speramtocytes- tests have shown that it is expressed in both mouse and human testis (3). It represents a new and unique type of potassium channel that is regulated by both intracellular pH and membrane voltage (1). Because of its sensitivity to both pH and voltage, Slo3 may play a role in alkalization-mediated K(+) fluxes associated with sperm capacitation (2).
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Bibliography
1. Schreiber M., et al. (1998) J Biol Chem. 273 (6): 3509-3516. 2. Tang Q.Y., Zhang Z., Xia X.M. and Lingle C.J. (2010) Epub: Channels (Austin) 4(1).
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Released date
13-Apr-2010
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_032458.3
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Gene number
16532
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Protein number
O54982
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody, Clone S2-16 (SMC-326). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody (SMC-326) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic, and some cell-cell border staining. Western Blot analysis of Rat brain membrane lysate showing detection of Slo3 Potassium Channel protein using Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody, Clone S2-16 (SMC-326). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody (SMC-326) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody, Clone S2-16 (SMC-326). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody (SMC-326) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermal Staining. Mouse Anti-Slo3 Potassium Channel Antibody [S2-16] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-326) Mouse Anti-Slo3 Potassium Channel Antibody [S2-16] used in Western Blot (WB) on Rat brain membrane lysate (SMC-326) Mouse Anti-Slo3 Potassium Channel Antibody [S2-16] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-326)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
