Mouse Anti-Mouse Slo3 Monoclonal IgG2b, Clone S2-16
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Catalog numberSMC-326D-A390
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Price:
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Size100 µg
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CloneS2-16
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ImmunogenFusion protein amino acids 1052-1121 of mouse Slo3
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Antibody s full descriptionMouse Anti-Mouse Slo3 Monoclonal IgG2b Antibody, Clone: S2-16: ATTO 390
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameKcnma3 Antibody, mSlo3 Antibody, Slo3 Antibody, KCa5 Antibody, KCNMA3 Antibody, potassium channel subfamily U member 1 Antibody, Slowpoke homolog 3 Antibody, KCa5.1 Antibody, KCNMC1Calcium-activated potassium channel Antibody, subfamily M subunit alpha-3 Antibody, Calcium-activated potassium channel subunit alpha-3 Antibody, potassium channel Antibody, subfamily U Antibody, member 1 Antibody
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Raised inMouse
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Antibody s targetSlo3
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Primary research fieldsNeuroscience, Ion Channels, Potassium Channels, Calcium-Activated Potassium Channels
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF , AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~115kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-326 was sufficient for detection of Slo3 in 10 µg of mouse brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe Slo3 channel is a novel potassium channel abundantly expressed in mammalian speramtocytes- tests have shown that it is expressed in both mouse and human testis (3). It represents a new and unique type of potassium channel that is regulated by both intracellular pH and membrane voltage (1). Because of its sensitivity to both pH and voltage, Slo3 may play a role in alkalization-mediated K(+) fluxes associated with sperm capacitation (2).
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Bibliography1. Schreiber M., et al. (1998) J Biol Chem. 273 (6): 3509-3516. 2. Tang Q.Y., Zhang Z., Xia X.M. and Lingle C.J. (2010) Epub: Channels (Austin) 4(1).
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Released date13-Apr-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_032458.3
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Gene number16532
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Protein numberO54982
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody, Clone S2-16 (SMC-326). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody (SMC-326) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic, and some cell-cell border staining. Western Blot analysis of Rat brain membrane lysate showing detection of Slo3 Potassium Channel protein using Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody, Clone S2-16 (SMC-326). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody (SMC-326) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody, Clone S2-16 (SMC-326). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody (SMC-326) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermal Staining. Mouse Anti-Slo3 Potassium Channel Antibody [S2-16] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-326) Mouse Anti-Slo3 Potassium Channel Antibody [S2-16] used in Western Blot (WB) on Rat brain membrane lysate (SMC-326) Mouse Anti-Slo3 Potassium Channel Antibody [S2-16] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-326)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolESB3, RPS2, PIRC83, PIRC88, IFNR, PIRC86
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Short nameMouse Anti-Mouse Slo3 Monoclonal IgG2b, Clone S2-16
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2b, IgG2b
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LabelATTO 390
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse Slo3 monoclonal IgG2b, clonality S2-16
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Alternative techniquemurine, antibodies
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Clone nameS2-16
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Gene info
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Identity
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Gene
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Long gene nameesterase B3
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Locus
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Discovery year2001-06-22
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameribosomal protein S2
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNA protein coding host genes
- S ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 83
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 88
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nameinterferon production regulator
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 86
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data