- Catalog numberSMC-326D-APC
- Product nameMouse Anti-Mouse Slo3 Monoclonal IgG2b, Clone S2-16
- Size100 µg
- PriceAsk For Price
- LabelAPC
- CloneS2-16
- ImmunogenFusion protein amino acids 1052-1121 of mouse Slo3
- Antibody s full descriptionMouse Anti-Mouse Slo3 Monoclonal IgG2b Antibody, Clone: S2-16: APC
- Antibody s categoryMonoclonal Antibodies
- Antibody s other nameKcnma3 Antibody, mSlo3 Antibody, Slo3 Antibody, KCa5 Antibody, KCNMA3 Antibody, potassium channel subfamily U member 1 Antibody, Slowpoke homolog 3 Antibody, KCa5.1 Antibody, KCNMC1Calcium-activated potassium channel Antibody, subfamily M subunit alpha-3 Antibody, Calcium-activated potassium channel subunit alpha-3 Antibody, potassium channel Antibody, subfamily U Antibody, member 1 Antibody
- Raised inMouse
- IsotypeIgG2b, IgG2b
- Antibody s targetSlo3
- Primary research fieldsNeuroscience, Ion Channels, Potassium Channels, Calcium-Activated Potassium Channels
- BrandnameNone
- Antibodies applicationsWB, IHC, ICC/IF , AM
- Antibody s reactivityHuman, Mouse, Rat
- Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
- PurityProtein G Purified
- Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~115kDa.
- Storage recommendations-20ºC
- Shipping recommendationsBlue Ice or 4ºC
- Antibody certificate of analysis1 µg/ml of SMC-326 was sufficient for detection of Slo3 in 10 µg of mouse brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Antibody in cellCell Membrane
- Tissue specificitySee included datasheet or contact our support service
- Scientific contextThe Slo3 channel is a novel potassium channel abundantly expressed in mammalian speramtocytes- tests have shown that it is expressed in both mouse and human testis (3). It represents a new and unique type of potassium channel that is regulated by both intracellular pH and membrane voltage (1). Because of its sensitivity to both pH and voltage, Slo3 may play a role in alkalization-mediated K(+) fluxes associated with sperm capacitation (2).
- Bibliography1. Schreiber M., et al. (1998) J Biol Chem. 273 (6): 3509-3516. 2. Tang Q.Y., Zhang Z., Xia X.M. and Lingle C.J. (2010) Epub: Channels (Austin) 4(1).
- Released date13-Apr-2010
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- NCBI numberNP_032458.3
- Gene number16532
- Protein numberO54982
- Antibody s datasheetContact our support service
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody, Clone S2-16 (SMC-326). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody (SMC-326) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic, and some cell-cell border staining. Western Blot analysis of Rat brain membrane lysate showing detection of Slo3 Potassium Channel protein using Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody, Clone S2-16 (SMC-326). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody (SMC-326) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody, Clone S2-16 (SMC-326). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Slo3 Potassium Channel Monoclonal Antibody (SMC-326) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermal Staining. Mouse Anti-Slo3 Potassium Channel Antibody [S2-16] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-326) Mouse Anti-Slo3 Potassium Channel Antibody [S2-16] used in Western Blot (WB) on Rat brain membrane lysate (SMC-326) Mouse Anti-Slo3 Potassium Channel Antibody [S2-16] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-326)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilityIn Stock
- Other related products
- Gene targetSlo3 Monoclonal IgG2b S2-16
- Identity:HGNC:10404
- Gene:RPS2
- Long gene name:ribosomal protein S2
- Synonyms:LLREP3S2
- Discovery year:1998-07-29
- Entrez gene record:6187
- Pubmed identification:9582194
- RefSeq identity:NM_002952
- Classification:Small nucleolar RNA protein coding host genes, S ribosomal proteins
- VEGA ID:OTTHUMG00000128708
- Identity:HGNC:37587
- Gene:PIRC83
- Long gene name:piwi-interacting RNA cluster 83
- Discovery year:2009-11-05
- Entrez gene record:100313891
- Pubmed identification:17881367
- Classification:Piwi-interacting RNA clusters
- Identity:HGNC:37592
- Gene:PIRC88
- Long gene name:piwi-interacting RNA cluster 88
- Discovery year:2009-11-05
- Entrez gene record:100313894
- Pubmed identification:17881367
- Classification:Piwi-interacting RNA clusters
- Identity:HGNC:37590
- Gene:PIRC86
- Long gene name:piwi-interacting RNA cluster 86
- Discovery year:2009-11-05
- Entrez gene record:100313911
- Pubmed identification:17881367
- Classification:Piwi-interacting RNA clusters
- Gene symbolESB3, RPS2, PIRC83, PIRC88, IFNR, PIRC86
- Hostmouse
- isotype filter
- IgG2b
- Short nameMouse Anti-Mouse Slo3 Monoclonal IgG2b, Clone S2-16
- label filter
- APC
- Clone nameS2-16
- technique filter
- anti-
- Mouse
- anti
- Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
- Alternative techniquemurine, antibodies