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About recombinant protein
CD30 ligand (CD30L) is a type-II membrane-associated glycoprotein protein belonging to the TNF superfamily and is expressed primarily on certain B cells, T cells, and monocytes. CD30L binds specifically to CD30 (receptor), which is expressed on activated, but not resting, B and T cells, in lymphomas and various chronically inflamed tissues. CD30L/CD30 interactions initiate a signaling cascade that can ultimately lead to the activation of NF-KappaB. CD30L/CD30 signaling exerts pleiotropic effects on normal cells, including cell death, differentiation, or cell division. Certain diseases, including Hodgkins’s lymphoma, allergic inflammation, diabetes (in NOD mice), and mycobacterial infection can also be affected by CD30L/CD30 signaling. The CD30L gene encodes for a 234 amino acid type II transmembrane protein, which contains a 37 amino acid cytoplasmic sequence, a 25 amino acid transmembrane domain and a 172 amino acid extracellular domain. Recombinant human soluble CD30L (sCD30L) is a 188 amino acid polypeptide corresponding to the extracellular domain, and contains an 8 residue N-terminal His -Tag.
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Other name of the recombinant
CD153, CD30L, CD30LG, Tumor necrosis factor ligand superfamily member 8, CD30 ligand, CD30-L
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Recombinant in
CHO cells
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SEQUENCE
HHHHHHHHPS PGGSGGQRTD SIPNSPDNVP LKGGNCSEDL LCILKRAPFK KSWAYLQVAK HLNKTKLSWN KDGILHGVRY QDGNLVIQFP GLYFIICQLQ FLVQCPNNSV DLKLELLINK HIKKQALVTV CESGMQTKHV YQNLSQFLLD YLQVNTTISV NVDTFQYIDT STFPLENVLS IFLYSNSD
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Activity
Biological activity was determined by its ability to stimulate human IL-8 production by human PBMC using a concentration range of 10.0-25.0 ng/mL. Note: Results may vary with PBMC donors. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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Keep at
The lyophilized sCD30 Ligand recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted sCD30 Ligand stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing sCD30 Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
4507607
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Accession nr
NP_001235.1
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Pureity
Greater than 95% by SDS-PAGE gel and HPLC analyses.
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About
This product is for research use only.
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Protein s origin
Human
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Vial
Lyophilized
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NCBI ID
944
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Gene name
tumor necrosis factor (ligand) superfamily, member 8
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Source
Homo sapiens
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Uniprot
P32971
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Description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
