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Synonyms
Soluble CD30 Ligand, TNFSF8, CD153, CD30L
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Alternative_names
Soluble CD30 Ligand, TNFSF8, CD153, CD30L
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Description
CD30L/CD30 interactions initiate a signaling cascade that lead to the activation of NF-Kappa B.
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Recombinant
Yes
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Source
CHO cells
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Purity by SDS PAGE
≥ 98%
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Assay
SDS-PAGE
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Purity
≥ 98%
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Biological activity
Determined by its ability to stimulate human IL-8 production by human PBMC using a concentration range of 10.0-25.0 ng/ml.
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Molecular Weight
21.3 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 1x PBS.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background Information
CD30 ligand (CD30L) is a type-II membrane-associated glycoprotein protein belonging to the TNF superfamily and is expressed primarily on certain B cells, T cells, and monocytes. CD30L binds specifically to CD30 (receptor), which is expressed on activated, but not resting, B and T cells, in lymphomas and various chronically inflamed tissues. CD30L/CD30 interactions initiate a signaling cascade that can ultimately lead to the activation of NF-Kappa B. CD30L/CD30 signaling exerts pleiotropic effects on normal cells, including cell death, differentiation, or cell division. Certain diseases, including Hodgkin’s lymphoma, allergic inflammation, diabetes (in NOD mice), and mycobacterial infection can also be affected by CD30L/CD30 signaling. The CD30L gene encodes for a 234 amino acid type II transmembrane protein, which contains a 37 amino acid cytoplasmic sequence, a 25 amino acid transmembrane domain and a 172 amino acid extracellular domain. Recombinant human soluble CD30L (sCD30L) is a 188 amino acid polypeptide corresponding to the extracellular domain, and contains an 8 residue N-terminal His -Tag.
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Amino acid sequence
HHHHHHHHP SPGGSGGQR TDSIPNSPD NVPLKGGNC SEDLLCILK RAPFKKSWA YLQVAKHLN KTKLSWNKD GILHGVRYQ DGNLVIQFP GLYFIICQL QFLVQCPNN SVDLKLELL INKHIKKQA LVTVCESGM QTKHVYQNL SQFLLDYLQ VNTTISVNV DTFQYIDTS TFPLENVLS IFLYSNSD
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
