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P53 Antibody / TP53

CAT: 0800-R20404-0.1MLSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-R20404-0.1MLSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody reacts to human p53 / Tumor protein 53 / TP53.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1:100-1:200, Western blot: 1:1000-1:2000
UniProt
P04637
Host
Rabbit
Reactivity
Human
Immunogen
A peptide corresponding to human p53 was used as the immunogen for the recombinant p53 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM387
Type
Recombinant
Applications
WB, IHC-P
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant p53 antibody at -20oC.
Limitations
This recombinant p53 antibody is available for research use only.
Storage Conditions
Store the recombinant p53 antibody at -20°C.
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant p53 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
Western blot testing of human HEK293 cell lysate with recombinant p53 antibody at 1:1000 dilution. Expected molecular weight ~53 kDa.

UniProtKB · P04637

Cellular tumor antigen p53

P53_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04637
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TP53
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q15086, Q15087, Q15088, Q16535, Q16807, Q16808, Q16809, Q16810, Q16811, Q16848, Q2XN98, Q3LRW1, Q3LRW2, Q3LRW3, Q3LRW4, Q3LRW5, Q86UG1, Q8J016, Q99659, Q9BTM4, Q9HAQ8, Q9NP68, Q9NPJ2, Q9NZD0, Q9UBI2, Q9UQ61
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinIsopeptide bondMethylationPhosphoproteinUbl conjugation

Molecular function

ActivatorDNA-bindingRepressor

Coding sequence diversity

Alternative promoter usageAlternative splicing

Biological process

ApoptosisBiological rhythmsCell cycleHost-virus interactionNecrosisTranscriptionTranscription regulation

Cellular component

CytoplasmCytoskeletonEndoplasmic reticulumMitochondrionNucleus

Disease

Disease variantLi-Fraumeni syndromeTumor suppressor

Ligand

Metal-bindingZinc
Product Datasheet
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