TP53 Antibody / p53

Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 protein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

9000-83-3

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

P04637

Human

Recombinant human full-length protein was used as the immunogen for the TP53 antibody.

RTP53/3889

Recombinant

IHC-P

Protein A/G affinity

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Aliquot the TP53 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

This TP53 antibody is available for research use only.

Aliquot the TP53 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the TP53 antibody should be determined by the researcher.

Nucleus