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TP53 Antibody / p53

CAT: 0800-V4440-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V4440-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. It is a tumor suppressor protein expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast, lung, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in many malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P04637
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the TP53 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RTP53/8579
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the TP53 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This TP53 antibody is available for research use only.
Storage Conditions
Aliquot the TP53 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the TP53 antibody should be determined by the researcher.
Location
Nucleus
Image Legend
IHC staining of FFPE human colon carcinoma tissue with TP53 antibody (clone rTP53/8579) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · P04637

Cellular tumor antigen p53

P53_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04637
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TP53
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q15086, Q15087, Q15088, Q16535, Q16807, Q16808, Q16809, Q16810, Q16811, Q16848, Q2XN98, Q3LRW1, Q3LRW2, Q3LRW3, Q3LRW4, Q3LRW5, Q86UG1, Q8J016, Q99659, Q9BTM4, Q9HAQ8, Q9NP68, Q9NPJ2, Q9NZD0, Q9UBI2, Q9UQ61
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinIsopeptide bondMethylationPhosphoproteinUbl conjugation

Molecular function

ActivatorDNA-bindingRepressor

Coding sequence diversity

Alternative promoter usageAlternative splicing

Biological process

ApoptosisBiological rhythmsCell cycleHost-virus interactionNecrosisTranscriptionTranscription regulation

Cellular component

CytoplasmCytoskeletonEndoplasmic reticulumMitochondrionNucleus

Disease

Disease variantLi-Fraumeni syndromeTumor suppressor

Ligand

Metal-bindingZinc

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