Recombinant AKT Antibody / PH domain

• Description:

  Akt isoforms are overexpressed in a variety of human tumors, and, at the genomic level, are amplified in gastric adenocarcinomas (Akt1), ovarian (Akt2), pancreatic (Akt2) and breast (Akt2) cancer. The role of Akt3 is less clear, though it appears to be predominantly expressed in the brain.
  
  Akt possesses a protein domain known as a PH domain, or Pleckstrin Homology domain, named after Pleckstrin, the protein in which it was first discovered. This domain binds to phosphoinositides with high affinity. In the case of the PH domain of Akt, it binds either PIP3 (phosphatidylinositol (3,4,5)-trisphosphate, PtdIns(3,4,5)P3) or PIP2 (phosphatidylinositol (3,4)-bisphosphate, PtdIns(3,4)P2).[9] This is useful for control of cellular signaling because the di-phosphorylated phosphoinositide PIP2 is only phosphorylated by the family of enzymes, PI 3-kinases (phosphoinositide 3-kinase or PI3-K), and only upon receipt of chemical messengers which tell the cell to begin the growth process. For example, PI 3-kinases may be activated by a G protein coupled receptor or receptor tyrosine kinase such as the insulin receptor. Once activated, PI 3-kinase phosphorylates PIP2 to form PIP3. [Wiki]

• Specifications:

  Immunohistochemistry (FFPE) : 1:200-1:500, Western Blot: 1:5000-1:10000

• UniProt:

  P31749, P31751, Q9Y243

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A peptide corresponding to the PH domain of human AKT1/2/3 was used as the immunogen for the recombinant AKT antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  RM316

• Type:

  Recombinant

• Applications:

  WB, IHC-P

• Purity:

  Protein A purified from animal origin-free supernatant

• Format:

  Purified

• Buffer:

  Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Reconstitution:

  Store the recombinant AKT antibody at -20oC.

• Limitations:

  This recombinant AKT antibody is available for research use only.

• Storage Conditions:

  Store the recombinant AKT antibody at -20°C.

• Formulation:

  Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the recombinant AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9000-83-3

• Location:

  Cytoplasmic, membranous, nuclear

• Image Legend:

  Western blot testing of human HEK293 cell lysate with recombinant AKT antibody at 1:10,000 dilution. Predicted molecular weight: ~56 kDa.