Recombinant EpCAM Antibody / Extracellular domain

• Description:

  EpCAM is frequently upregulated in carcinomas but is not expressed in cancers of non-epithelial origin. EpCAM expression is not limited to human colon carcinomas; in fact, EpCAM is expressed in a variety of human epithelial tissues, carcinomas, and progenitor and stem cells. [Wiki]

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  P16422

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  EPM17-5R

• Type:

  Recombinant

• Applications:

  IHC-P, WB

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant EpCAM antibody is available for research use only.

• Storage Conditions:

  Store the recombinant EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9000-83-3

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  Western blot testing of human lung lysate with EpCAM antibody (clone EPM17-5R) . Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated) .