Recombinant EpCAM Antibody / Cytoplasmic domain

• Description:

  Epithelial cellular adhesion molecule (Ep-CAM), also called epithelial specific antigen (ESA), is a 40-43kDa transmembrane epithelial glycoprotein. It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P16422

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 289-314 of human EpCAM was used as the immunogen for the recombinant EpCAM antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  EGP40/7035R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the recombinant EpCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This recombinant EpCAM antibody is available for research use only.

• Storage Conditions:

  Aliquot the recombinant EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  IHC staining of FFPE human colon tissue with recombinant EpCAM antibody (clone EGP40/7035R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.