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ELISA Blocking Buffer

CAT: 0246-642Size: 500 mLDry Ice: NoHazardous: No
CAT#:0246-642Size:500 mL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Eliminate interference and nonspecific background associated with antibody-coated ELISA formats and sandwich ELISAs. ELISA Blocking Buffer (formerly known as Synblock) provides low background performance without the use of conventional cross-reactive protein additives.
CAS Number
7732-18-5
Assay Principle
1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer., 2. Incubate 8–24 hours at room temperature (RT)., 3. Aspirate the coating solution., 4. Wash each well twice with ICT’s ELISA Wash Buffer., 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution., 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT., 7. Aspirate the blocking buffer; do not wash., 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.
Concentration
5X
pH
pH: 7.4 at 1X
Shipping Conditions
Blue Ice
Storage Conditions
4°C
Shelf Life
24 months from date of manufacture

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