Products for Research Use Only
RegisterLogin

ELISA Blocking Buffer

CAT: 0246-642Size: 500 mLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0246-642Size:500 mL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Eliminate interference and nonspecific background associated with antibody-coated ELISA formats and sandwich ELISAs. ELISA Blocking Buffer (formerly known as Synblock) provides low background performance without the use of conventional cross-reactive protein additives.
CAS Number
7732-18-5
Assay Principle
1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer., 2. Incubate 8–24 hours at room temperature (RT)., 3. Aspirate the coating solution., 4. Wash each well twice with ICT’s ELISA Wash Buffer., 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution., 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT., 7. Aspirate the blocking buffer; do not wash., 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.
Concentration
5X
pH
pH: 7.4 at 1X
Shipping Conditions
Blue Ice
Storage Conditions
4°C
Shelf Life
24 months from date of manufacture

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

Related Products

CatalogName
650ELISA Wash Buffer, 10X
652ELISA Wash Buffer, 10X
651ELISA Wash Buffer, 10X
AR-6567-02Peroxidase Stabilizing Buffer
AR-6544-02Citrate Buffer, 10X
AR-6565-02Phosphate Buffer, 10X
AR-6565-05Phosphate Buffer, 10X
AR-6545-05EDTA Buffer, 10X
AR-6544-05Citrate Buffer, 10X
AR-6567-03Peroxidase Stabilizing Buffer

Alternative Products

CatalogName
E104ELISA Blocking Buffer
GWB-E120C6Blocking Buffer for ELISA
NE053728102Easy ELISA Blocking Buffer
MBS2571969-01ELISA Plate Blocking Buffer
GTR19288334SynBlock ELISA Blocking Buffer
BUF81201-100PanBlock ELISA Blocking Buffer
OORA00238-1LELISA Microwell Blocking Buffer with Stabilizer
GTR19334027General Block ELISA Blocking Buffer
6301Alternative Block ELISA Blocking Buffer
MBS258384-01Neptune Block ELISA Blocking Buffer

Popular Products